Moyamoya is a cerebrovascular disorder characterized by progressive stenosis of the intracranial internal carotid arteries. There are two forms: Disease and Syndrome, with each characterized by the sub-population it affects. Moyamoya syndrome (MMS) is more prominent in adults in their 20’s-40’s, and is often associated with autoimmune diseases. Currently, there are no surgical models for inducing moyamoya syndrome, so our aim was to develop a new animal model to study this relatively unknown cerebrovascular disease. Here, we demonstrate a new surgical technique termed internal carotid artery stenosis (ICAS), to mimic MMS using micro-coils on the proximal ICA. We tested for Moyamoya-like vasculopathies by fluorescently labelling the mouse cerebrovasculature with Di I for visualization and analysis of vessel diameter at the distal ICA and anastomoses on the cortical surface. Results show a significant narrowing of the distal ICA and anterior cerebral artery (ACA) in the Circle of Willis, as observed in humans. There is also a significant decrease in the number of anastomoses between the middle cerebral artery (MCA) and the ACA in the watershed region of the cortex. While further characterization is needed, this ICAS model can be applied to transgenic mice displaying co-morbidities as observed within the Moyamoya syndrome population, allowing a better understanding of the disease and development of novel treatments.

Moyamoya是一种以颅内颈内动脉进行性狭窄为特征的脑血管疾病。有两种形式：疾病和综合症，每种形式都以其影响的亚群为特征。Moyamoya综合征（MMS）在成年人中20至40年代更为突出，通常与自身免疫性疾病相关。目前，尚无诱发烟雾病综合征的手术模型，因此我们的目标是开发一种新的动物模型来研究这种相对未知的脑血管疾病。在这里，我们演示了一种称为颈内动脉狭窄（ICAS）的新手术技术，该技术可使用近端ICA上的微线圈来模仿MMS。我们通过用Di I荧光标记小鼠脑血管系统来观察和分析Moyamoyaya样血管病变，以可视化和分析ICA远端的血管直径和皮质表面的吻合口。结果表明，在人类中观察到，威利斯圆环中的远端ICA和前脑动脉（ACA）明显变窄。大脑中动脉（MCA）与皮质分水岭区域中的ACA之间的吻合数目也显着减少。尽管还需要进一步的表征，但这种ICAS模型可以应用于显示出Moyamoya综合征人群中观察到的合并症的转基因小鼠，从而可以更好地了解疾病并开发新的治疗方法。结果表明，在人类中观察到，威利斯圆环中的远端ICA和前脑动脉（ACA）明显变窄。大脑中动脉（MCA）与皮质分水岭区域中的ACA之间的吻合数目也显着减少。尽管还需要进一步的表征，但这种ICAS模型可以应用于显示出Moyamoya综合征人群中观察到的合并症的转基因小鼠，从而可以更好地了解疾病并开发新的治疗方法。结果表明，在人类中观察到，威利斯圆环中的远端ICA和前脑动脉（ACA）明显变窄。大脑中动脉（MCA）与皮质分水岭区域中的ACA之间的吻合数目也显着减少。尽管还需要进一步的表征，但这种ICAS模型可以应用于显示出Moyamoya综合征人群中观察到的合并症的转基因小鼠，从而可以更好地了解疾病并开发新的治疗方法。