Novel mechanically reinforced nanocomposite hydrogels (NCHs) were developed based on methacrylated gellan gum (MGG) and cationic polyurethane nanoparticles (CPUNs) through a green chemical approach. A series of NCHs were synthesized by the incorporation of CPUNs with weight ratios of 0, 10, 30 and 50 w/w % into the MGG solution, with two different methacrylation degrees (1.2, 5.6%). The chemical structure, morphology, mechanical properties, stimuli-responsivity and cytotoxicity of synthesized NCHs were investigated. Analysis of the hydrogels mechanical testing demonstrated that the addition of CPUNs affords the significant increase in compressive properties. Meanwhile, the formulation of NCH containing the MGG with lower methacrylation degree and 30 w/w % CPUNs showed the highest mechanical properties. Furthermore, equilibrium swelling ratio of the hydrogels decreased by CPUNs addition. Finally, it is worth mentioning that NCHs showed no significant toxicity to human dermal fibroblast cells (HDFs) which idealize them as the suitable hydrogels for biomedical applications.

通过绿色化学方法，开发了基于甲基丙烯酸吉兰糖胶（MGG）和阳离子聚氨酯纳米粒子（CPUNs）的新型机械增强纳米复合水凝胶（NCH）。通过将重量比为0、10、30和50 w / w％的CPUN掺入MGG溶液中，合成了一系列NCH，具有两种不同的甲基丙烯酸化度（1.2、5.6％）。研究了合成NCHs的化学结构，形态，力学性能，刺激反应性和细胞毒性。对水凝胶力学测试的分析表明，添加CPUN可以显着提高压缩性能。同时，含有较低甲基丙烯酸酯化度和30 w / w％CPUNs的MGG的NCH配方具有最高的机械性能。此外，添加CPUNs降低了水凝胶的平衡溶胀率。最后，值得一提的是，NCHs对人皮肤成纤维细胞（HDF）没有明显的毒性，使它们理想化为适合生物医学应用的水凝胶。