A novel natural bioflocculant resource of Ruditapes philippinarum conglutination mud (RPM) with the effective flocculation components was firstly reported in this study. Experimental results showed that the maximum flocculation activity (FR) of RPM to kolin clay reached 86.7% in deionized water assay system and 91.8% in sea water assay system. RPM could flocculate marine microalgae Chlorella salina with a FR of 74.1%. The crude RPM polysaccharides extract(RPMP) were composed of 97.8% (w/w) polysaccharides and 2.2% (w/w) protein and the functional components of pure RPMP were firstly discovered to be two complex heteropolysaccharides of RPMP-1 and RPMP-2 with similar monosaccharides composition except glucose content. The molecular weights of RPMP-1 and RPMP-2 were 5.7 kDa and 18.0 kDa, respectively. It is interesting to find that RPMP-1 and RPMP-2 exhibited synergistic flocculation activity of 65.6% at a mass ratio of 2:1 as in crude RPMP, suggesting the original proportion is significant to synergistic flocculation.

本研究首次报道了具有有效絮凝成分的菲律宾蛤仔凝集泥（RPM）的新型天然生物絮凝剂资源。实验结果表明，去离子水测定系统中RPM对高岭土的最大絮凝活性达到86.7％，海水测定系统中达到91.8％。RPM可絮凝海洋微藻小球藻FR为74.1％。RPM粗多糖提取物（RPMP）由97.8％（w / w）多糖和2.2％（w / w）蛋白质组成，并且首先发现纯RPMP的功能成分是RPMP-1和RPMP-的两种复杂杂多糖。 2具有相似的单糖组成，但葡萄糖含量除外。RPMP-1和RPMP-2的分子量分别为5.7 kDa和18.0 kDa。有趣的是，发现RPMP-1和RPMP-2的质量比为1：1，与粗制RPMP相比，具有65.6％的协同絮凝活性，这表明原始比例对协同絮凝具有重要意义。