Copper is an essential trace metal for different physiological processes in phytoplankton, being either a limiting or toxic element depending on its bioavailability, which may induce local physiological adaptations. Atmospheric Cu deposition to the oceans can negatively impact phytoplankton growth, with the most Cu-sensitive phytoplankton exhibiting differences based on coastal vs oceanic origin. The goal of this work was to analyze sensitivity to Cu toxicity of the cosmopolitan marine calcifying phytoplankton, Emiliania huxleyi, exploring what factors determine intraspecific variability in sensitivity. We compared 17 strains isolated from coastal and open ocean waters of the Eastern South Pacific (ESP), the Mediterranean Sea, and the Tasman Sea. Offshore strains were as sensitive to Cu than coastal strains. Sensitivity to Cu was explained well by predicted depositional inputs of atmospheric Cu in the ESP both for coastal and offshore strains, but not when considered globally. The variability in Cu-sensitivity was also due to the production of organic Cu-ligands (CL), being the most productive strains the most tolerant to Cu at constitutive levels. When exposed to 100 nM Cu, E. huxleyi produced significantly higher amounts of CL, especially coastal strains, but CL production did not correlate to observed EC50s.

铜是浮游植物不同生理过程必不可少的微量金属，取决于其生物利用度，铜可能是限制性元素或有毒元素，可能会引起局部生理适应。大气中铜在海洋中的沉积会对浮游植物的生长产生负面影响，对铜最敏感的浮游植物表现出基于沿海和海洋起源的差异。这项工作的目的是分析大洋海洋钙化浮游植物Emililiania huxleyi对Cu毒性的敏感性。，探索哪些因素决定种内敏感性的变异性。我们比较了从东太平洋（ESP），地中海和塔斯曼海的沿海和开放海域分离出的17种菌株。海上菌株对铜的敏感性比沿海菌株高。通过对沿海和近海菌株在ESP中大气Cu的预测沉积输入，可以很好地解释对Cu的敏感性，但在全球范围内则不能。Cu敏感性的差异还归因于有机Cu-配体（CL）的产生，其是在组成水平上对Cu最耐受的最有生产力的菌株。当暴露于100  nM Cu时，虎肠埃希氏菌产生的CL量要高得多，尤其是沿海菌株，但CL的产生与观察到的EC50无关。