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Fluorescent Diarylethene Photoswitches—A Universal Tool for Super‐Resolution Microscopy in Nanostructured Materials
Small ( IF 13.0 ) Pub Date : 2018-01-11 , DOI: 10.1002/smll.201703333 Oleksii Nevskyi 1 , Dmytro Sysoiev 2 , Jes Dreier 3 , Simon Christoph Stein 4 , Alex Oppermann 1 , Florian Lemken 1 , Tobias Janke 1 , Jörg Enderlein 4 , Ilaria Testa 3 , Thomas Huhn 2 , Dominik Wöll 1
Small ( IF 13.0 ) Pub Date : 2018-01-11 , DOI: 10.1002/smll.201703333 Oleksii Nevskyi 1 , Dmytro Sysoiev 2 , Jes Dreier 3 , Simon Christoph Stein 4 , Alex Oppermann 1 , Florian Lemken 1 , Tobias Janke 1 , Jörg Enderlein 4 , Ilaria Testa 3 , Thomas Huhn 2 , Dominik Wöll 1
Affiliation
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Super‐resolution fluorescence microscopy allows for unprecedented in situ visualization of biological structures, but its application to materials science has so far been comparatively limited. One of the main reasons is the lack of powerful dyes that allow for labeling and photoswitching in materials science systems. In this study it is shown that appropriate substitution of diarylethenes bearing a fluorescent closed and dark open form paves the way for imaging nanostructured materials with three of the most popular super‐resolution fluorescence microscopy methods that are based on different concepts to achieve imaging beyond the diffraction limit of light. The key to obtain optimal resolution lies in a proper control over the photochemistry of the photoswitches and its adaption to the system to be imaged. It is hoped that the present work will provide researchers with a guide to choose the best photoswitch derivative for super‐resolution microscopy in materials science, just like the correct choice of a Swiss Army Knife's tool is essential to fulfill a given task.
中文翻译：
荧光二芳基乙烯光电开关—纳米结构材料中超高分辨率显微镜的通用工具
超分辨率荧光显微镜可以实现生物结构的前所未有的原位可视化，但是迄今为止它在材料科学中的应用受到了相对的限制。主要原因之一是缺乏能在材料科学系统中进行标记和光转换的强力染料。在这项研究中表明，带有荧光封闭和暗开口形式的二芳烃的适当取代为使用三种最流行的超分辨率荧光显微镜方法成像纳米结构材料铺平了道路，这三种方法基于不同的概念来实现衍射以外的成像光的极限。获得最佳分辨率的关键在于对光电开关的光化学及其对要成像系统的适应性的适当控制。
更新日期：2018-01-11
中文翻译：
荧光二芳基乙烯光电开关—纳米结构材料中超高分辨率显微镜的通用工具
超分辨率荧光显微镜可以实现生物结构的前所未有的原位可视化，但是迄今为止它在材料科学中的应用受到了相对的限制。主要原因之一是缺乏能在材料科学系统中进行标记和光转换的强力染料。在这项研究中表明，带有荧光封闭和暗开口形式的二芳烃的适当取代为使用三种最流行的超分辨率荧光显微镜方法成像纳米结构材料铺平了道路，这三种方法基于不同的概念来实现衍射以外的成像光的极限。获得最佳分辨率的关键在于对光电开关的光化学及其对要成像系统的适应性的适当控制。
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