Particles which bear tumor cell targeting and multimode imaging capabilities are promising in tumor diagnosis and cancer therapy. A simple and versatile method to fabricate gold/polydopamine-Methylene [email protected] Serum Albumin–glutaraldehyde–Transferrin composite particles (Au/[email protected] NPs) for tumor cell targeting and fluorescence (FL) / surface-enhanced Raman scattering (SERS) dual-modal imaging were reported in this work. Polydopamine (PDA) spheres played an important role in gold ion reduction, gold nanoparticle (Au NPs) binding and methylene blue (MB) adsorption, MB were employed as both fluorescence label and Raman reporter. In addition, glutaraldehyde (GA) crosslinked bovine serum albumin (BSA) in the outer layer of Au/PDA-MB nanoparticles can prevent MB from dissociation and leakage. The composite nanoparticles were further conjugated with transferrin (Tf) to target transferrin receptor (TfR)-overexpressed cancer cells. The targeting ability as well as the intracellular location of the probe was investigated through SERS mapping and fluorescence imaging. Their excellent biocompatibility was demonstrated by low cytotoxicity against breast cancer cell (4T1 cell).

具有肿瘤细胞靶向和多模成像能力的颗粒在肿瘤诊断和癌症治疗中很有前途。一种简单通用的金/聚多巴胺-亚甲基[受电子邮件保护的]血清白蛋白-戊二醛-转铁蛋白复合颗粒（Au / [受电子邮件保护的] NPs）的制备方法，用于靶向肿瘤细胞和荧光（FL）/表面增强拉曼散射（SERS） ）双模态成像在这项工作中报告。聚多巴胺（PDA）球在金离子还原，金纳米颗粒（Au NPs）结合和亚甲基蓝（MB）吸附中起着重要作用，MB被用作荧光标记和拉曼报道分子。此外，Au / PDA-MB纳米颗粒外层中的戊二醛（GA）交联的牛血清白蛋白（BSA）可以防止MB解离和泄漏。将复合纳米颗粒进一步与转铁蛋白（Tf）偶联，以靶向过表达转铁蛋白受体（TfR）的癌细胞。通过SERS作图和荧光成像研究了探针的靶向能力以及细胞内位置。它们对乳腺癌细胞（4T1细胞）的细胞毒性低，证明了其优异的生物相容性。