A “turn-on” fluorescence sensor for ascorbic acid based on graphene quantum dots via fluorescence resonance energy transfer†,Analytical Methods

当前位置： X-MOL 学术 › Anal. Methods › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A “turn-on” fluorescence sensor for ascorbic acid based on graphene quantum dots via fluorescence resonance energy transfer†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-01-11 00:00:00 , DOI: 10.1039/c7ay02828h
Yue Gao _{1,

2,

3,

4,

5} , Xiaolu Yan _{1,

2,

3,

4,

5} , Meng Li _{1,

2,

3,

4,

5} , Han Gao _{1,

2,

3,

4,

5} , Jing Sun _{5,

6,

7,

8,

9} , Shuyun Zhu _{1,

2,

3,

4,

5} , Shuang Han _{5,

10,

11,

12} , Li-Na Jia _{1,

2,

3,

4,

5} , Xian-En Zhao _{1,

2,

3,

4,

5} , Hua Wang _{1,

2,

3,

4,

5}

Affiliation

A novel turn-on fluorescent sensor for the detection of ascorbic acid (AA) has been developed based on the fluorescence resonance energy transfer (FRET) between graphene quantum dots (GQDs) and squaric acid (SQA)-iron(III). In this assay, iron(III) can rapidly coordinate with the SQA to produce SQA-iron(III). The absorbance band of SQA-iron(III) could be largely overlapped with the emission band of GQDs, thus resulting in the fluorescence resonance energy transfer (FRET)-induced fluorescence quenching of GQDs. Moreover, the fluorescence of GQDs can be sensitively turned on by AA through the oxidation-reduction between iron(III) and AA. After the transformation of SQA-iron(III) to SQA-iron(II), because the absorbance of the solution reduces, the FRET decreases. This FRET-based nanosensor shows high selective and sensitive response in the concentration of AA ranging from 1.0 to 95 μM with the detection limit as lower as 200 nM, which is lower than that of other fluorescent assays. Finally, the proposed sensing system was successfully applied to the direct analysis of AA in the real samples with satisfactory results.

中文翻译：

基于石墨烯量子点的抗坏血酸“开启”荧光传感器，通过荧光共振能量转移来实现†

基于石墨烯量子点（GQDs）和方酸（SQA）-铁（III）之间的荧光共振能量转移（FRET），开发了一种用于检测抗坏血酸（AA）的新型开启式荧光传感器。在该测定中，铁（III）可以与SQA快速配位以产生SQA-铁（III）。SQA-铁（III）的吸收带可能与GQDs的发射带有很大的重叠，从而导致荧光共振能量转移（FRET）引起GQDs的荧光猝灭。此外，通过铁（III）与AA之间的氧化还原，AA可以灵敏地开启GQDs的荧光。SQA-铁（III）改造后）至SQA-铁（II），因为溶液的吸光度降低，所以FRET降低。这种基于FRET的纳米传感器在AA浓度范围为1.0至95μM时显示出高选择性和灵敏响应，其检测限低于200 nM，低于其他荧光测定法。最后，所提出的传感系统已成功应用于实际样品中AA的直接分析，结果令人满意。

更新日期：2018-01-11

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11