A New Fluorogenic Probe for the Detection of endo‐β‐N‐Acetylglucosaminidase,ChemBioChem

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A New Fluorogenic Probe for the Detection of endo‐β‐N‐Acetylglucosaminidase
ChemBioChem ( IF 2.6 ) Pub Date : 2018-02-16 , DOI: 10.1002/cbic.201700662
Nozomi Ishii ₁ , Chie Sunaga ₁ , Kanae Sano ₁ , Chengcheng Huang ₂ , Kenta Iino ₃ , Yuji Matsuzaki ₃ , Tadashi Suzuki ₂ , Ichiro Matsuo ₁

Affiliation

Real‐time monitoring of ENGase activity: A fluorescence‐quenching‐based assay system to determine the hydrolysis activity of endo‐β‐N‐acetylglucosaminidase (ENGase) was developed. We succeeded in measuring the hydrolysis activities of ENGases quantitatively. This assay system is suitable for screening potential inhibitors of human ENGase, which could serve as therapeutic agents for N‐glycanase 1 (NGLY1) deficiency.

中文翻译：

一种新型荧光探针，用于检测内-β-N-乙酰氨基葡糖苷酶

实时监测ENGase活性：开发了一种基于荧光猝灭的测定系统，用于测定β- N-乙酰基氨基葡糖苷内切酶（ENGase）的水解活性。我们成功地定量测量了ENGases的水解活性。该测定系统适用于筛选人ENGase的潜在抑制剂，该抑制剂可作为N-聚糖酶1（NGLY1）缺乏症的治疗剂。

更新日期：2018-02-16

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