AbstractThe authors describe a lateral flow assay (LFA) for the antibody against the infectious bacterium Clostridium tetani. Gold nanoparticles (AuNPs) were linked to tetanus antigen and are captured in the test line via the formation of a sandwich structure composed of AuNP-labeled tetanus antigen, tetanus antibody, and tetanus antigen. This leads to the formation of a characteristic red line due to the accumulation of AuNPs. The formation of the color line allows for a highly sensitive and selective detection of tetanus antibody, both with bare eyes and by smartphone-based quantitative analysis. This assay offers a wide detection range from 0 to 0.5 IU·mL−1 and has a linear relationship from 0.01 to 0.1 IU·mL−1 with an experimental detection limit of 0.01 IU·mL−1. This assay is simple, fast, inexpensive and highly selective. When applied to the detection of tetanus antibody in spiked whole blood, it provided reliable results that compared well to those obtained with a commercial ELISA kit. Graphical abstractᅟ

中文翻译：

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金纳米颗粒标记破伤风抗原测定全血中人破伤风抗体的侧向流法

摘要作者描述了一种针对感染性细菌破伤风梭菌的抗体的横向流动测定 (LFA)。金纳米粒子 (AuNPs) 与破伤风抗原相连，并通过形成由 AuNP 标记的破伤风抗原、破伤风抗体和破伤风抗原组成的夹心结构被捕获在测试线中。由于 AuNPs 的积累，这导致形成特征红线。颜色线的形成允许通过裸眼和基于智能手机的定量分析对破伤风抗体进行高灵敏度和选择性检测。该测定提供了从 0 到 0.5 IU·mL-1 的广泛检测范围，并且在 0.01 到 0.1 IU·mL-1 之间具有线性关系，实验检测限为 0.01 IU·mL-1。该测定简单、快速、廉价且选择性高。当应用于加标全血中破伤风抗体的检测时，它提供了可靠的结果，与使用商业 ELISA 试剂盒获得的结果相比较。图形摘要ᅟ