In this study, the electrochemical properties of a Shewanella oneidensis MR-1 biofilm were investigated using a mini-microbial electrochemical system. The performance of the biofilm was shown, using discharge test and cyclic voltammetry investigations, to improve over time. Differential pulse voltammograms were also acquired to determine the type of extracellular electron transfer that took place and to characterize the structure of the microbial biofilm formed on the electrode of the electrochemical system. These results indicated that extracellular electron transfer via a flavin-like mediator chemical predominated as the biofilm grew. The results, combined with a comparison of the measured current density with the calculated value of a seamless single-layered biofilm, also suggested that S. oneidensis MR-1 formed a multi-layered biofilm on the electrode.

在这项研究中，使用微型微生物电化学系统研究了Shewanella oneidensis MR-1生物膜的电化学特性。使用放电测试和循环伏安法研究显示了生物膜的性能，可随着时间的推移而改善。还获得了差分脉冲伏安图，以确定发生的细胞外电子转移的类型，并表征在电化学系统电极上形成的微生物生物膜的结构。这些结果表明细胞外电子通过随着生物膜的生长，黄素类介体化学物质占主导地位。结果与测量的电流密度与无缝单层生物膜的计算值的比较相结合，也表明沙门氏菌MR-1在电极上形成了多层生物膜。