Herein, we present a novel sandwich fluorescence enzyme linked immunosorbent assay (ELISA) for highly sensitive detection of Hepatitis B virus surface antigen (HBsAg) based on glucose oxidase (GOx)-induced fluorescence quenching of mercaptopropionic acid-modified CdTe quantum dots (MPA-QDs). In this system, hydrogen peroxide (H₂O₂) sensitive MPA-QDs was used as a signal output, and glucose oxidase (GOx) was used as label which can generate H₂O₂ via catalytic oxidation of glucose. The proposed method showed dynamic linear detection of HBsAg both in the range of 47 pg mL⁻¹ ~ 380 pg mL⁻¹ and 0.75 ng mL⁻¹ ~ 12.12 ng mL⁻¹. The detection limit of the proposed fluorescence ELISA was 1.16 pg mL⁻¹, which was approximately 430-fold lower than that of horseradish peroxidase (HRP)-based conventional ELISA. The average recoveries for HBsAg-spiked serum samples ranged from 98.0% to 126.8% with the relative standard derivation below 10%, thus indicating acceptable precision and high reproducibility of the proposed fluorescence ELISA for HBsAg detection. Additionally, the developed method showed no false positive results analyzing 35 real HBsAg-negative serum samples, and exhibited excellent agreement (R²=0.9907) with a commercial time-resolved fluorescence immunoassay (TRFIA) kit for detecting 31 HBsAg-positive serum samples. In summary, the proposed method based on fluorescence quenching of H₂O₂ sensitive QDs is considerably to be an excellent biodetection platform with ultrahigh sensitivity, good accuracy and excellent reliability.

在这里，我们提出了一种新型的三明治荧光酶联免疫吸附测定（ELISA），用于基于葡萄糖氧化酶（GOx）诱导的巯基丙酸修饰的CdTe量子点（MPA- QDs）。在该系统中，使用过氧化氢（H ₂ O ₂）敏感的MPA-QDs作为信号输出，使用葡萄糖氧化酶（GOx）作为可通过葡萄糖催化氧化生成H ₂ O _2的标记。所提出的方法表现出的HBsAg的动态线性检测两者是否在范围内47皮克毫升^-1〜380皮克毫升^-1和0.75纳克毫升^-1〜12.12纳克毫升^-1。拟议的荧光ELISA的检测限为1.16 pg mL ^-1，比基于辣根过氧化物酶（HRP）的常规ELISA的检测限低约430倍。掺加HBsAg的血清样品的平均回收率在98.0％至126.8％之间，相对标准偏差低于10％，因此表明所提出的用于HBsAg检测的荧光ELISA的精密度和可重复性很高。此外，开发的方法在分析35个真实的HBsAg阴性血清样品中没有显示假阳性结果，并且与用于检测31个HBsAg阳性血清样品的商业时间分辨荧光免疫分析（TRFIA）试剂盒显示出极好的一致性（R ² = 0.9907）。综上所述，所提出的基于H ₂荧光猝灭的方法O ₂敏感的QD可以成为具有超高灵敏度，良好准确性和出色可靠性的出色生物检测平台。