A CSTR reactor was operated for 30 days. It was fed with raw cheese whey with an organic loading rate of 30 gCOD/Ld. Hydrogen production varied significantly with a maximum of 0.9 LH₂/Ld and decreased after 17 days of operation. The causes of production instability were analysed using different microbiological tools. It was concluded that this decrease was not due to the incapacity to select hydrogen-producing organisms, as shown by the persistence of the Fe-hydrogenase genes in the reactor. Using a molar balance, it was estimated that more than 30% of the acetic production can be due to the homoacetogenesis pathway, although genes from homoacetogenic microorganisms were detected at a very low concentration. The different effects of enhancement and inhibition of hydrogen production by the lactic acid bacteria and their high abundance variation could explain the instability of hydrogen production in this reactor.

CSTR反应器运行30天。用有机奶酪装载量为30 gCOD / Ld的原始干酪乳清喂养。氢气产量变化很大，最大为0.9 LH ₂/ Ld，并在手术17天后下降。使用不同的微生物工具分析了生产不稳定的原因。结论是，这种减少不是由于不能选择产氢生物，如反应堆中铁氢酶基因的持久性所表明的那样。使用摩尔平衡，虽然来自同产乙酸微生物的基因被检测到的浓度非常低，但据估计，超过30％的产乙酸可能归因于同产乙酸途径。乳酸菌增强和抑制产氢的不同作用及其高丰度变化可以解释该反应器中产氢的不稳定性。