A purely DNA nanomachine must support internal stresses across short DNA segments with finite rigidity, producing effects that can be qualitatively very different from experimental observations of isolated DNA in fixed-force ensembles. In this article, computational simulations are used to study how well the rigidity of a driving DNA duplex can rupture a double-stranded DNA target into single-stranded segments and how well this stress can discriminate between unzipping or shearing geometries. This discrimination is found to be maximized at an optimal length but deteriorates as the driving duplex is either lengthened or shortened. This differs markedly from a fixed-force ensemble and has implications for the design parameters and limitations of dynamic DNA nanomachines.

中文翻译：

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DNA破裂DNA的能力如何？DNA纳米结构内部的剪切力和拉开力

纯粹的DNA纳米机械必须以有限的刚度支撑短的DNA片段内的内应力，其产生的质量与定力合奏中分离的DNA的实验观察在质量上有很大不同。在本文中，使用计算机模拟来研究驱动DNA双链体的刚性如何将双链DNA靶断裂成单链段，以及该应力如何区分拉开或剪切的几何形状。发现这种区分在最佳长度上最大化，但是随着加长或缩短驱动双工而恶化。这与固定力合奏明显不同，并且对动态DNA纳米机器的设计参数和局限性有影响。