Multifunctional Poly‐N‐Isopropylacrylamide/DNAzyme Microgels as Highly Efficient and Recyclable Catalysts for Biosensing,Advanced Functional Materials

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Multifunctional Poly‐N‐Isopropylacrylamide/DNAzyme Microgels as Highly Efficient and Recyclable Catalysts for Biosensing
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2018-01-10 , DOI: 10.1002/adfm.201705876
Fengyun Li ₁ , Chunyan Wang ₁ , Weiwei Guo ₁

Affiliation

The development of highly efficient, recyclable, and multifunctional biocatalysts is of great importance for various applications, especially in biosensing. In this study, highly catalytic and recyclable DNAzyme functionalized poly‐N‐isopropylacrylamide (pNIPAM) microgels are prepared via one‐step precipitation polymerization. The pNIPAM/DNAzyme microgels exhibit highly catalytic activities in aqueous solution at room temperature, and become hydrophobic and separable from the reaction mixture at temperature higher than the lower critical solution temperature of pNIPAM, which facilitate the recyclable utilization of these catalysts. Different kinds of DNAzyme functionalized catalytic microgels can be facilely prepared via the one‐step synthesis procedure. Two typical catalytic DNA structures, the Mg²⁺‐dependent DNAzyme and the hemin‐G‐quadruplex horseradish peroxidase (HRP)‐mimicking DNAzyme, are chosen as model systems to validate the feasibility. These pNIPAM/DNAzyme microgel catalysts maintain 80% to 91% initial catalytic activity after eight times of catalysis recycling. Furthermore, the pNIPAM microgels by themselves provide additional interfaces to capturing an enzyme, glucose oxidase, which can cascade with the linked HRP mimicking DNAzymes, to form recyclable bi‐enzyme cascading system for the sensing of glucose.

中文翻译：

多功能聚-N-异丙基丙烯酰胺/ DNAzyme微凝胶是高效且可回收的生物传感催化剂

高效，可回收和多功能的生物催化剂的开发对于各种应用，特别是在生物传感领域具有重要意义。在这项研究中，通过一步沉淀聚合制备了高度催化和可回收利用的DNAzyme功能化聚N-异丙基丙烯酰胺（pNIPAM）微凝胶。pNIPAM / DNAzyme微凝胶在室温下在水溶液中表现出高度的催化活性，并且在高于pNIPAM的较低临界溶液温度的温度下变得疏水且可与反应混合物分离，这有利于这些催化剂的可循环利用。可通过一步合成程序轻松制备各种类型的DNAzyme功能化催化微凝胶。Mg是两种典型的催化DNA结构^选择2+依赖性DNAzyme和模仿hemin-G-quadruplex辣根过氧化物酶（HRP）的DNAzyme作为模型系统以验证可行性。这些pNIPAM / DNAzyme微凝胶催化剂经过八次催化循环后，仍保持80％至91％的初始催化活性。此外，pNIPAM微凝胶本身提供了捕获酶（葡萄糖氧化酶）的附加接口，该酶可以与模拟DNAzyme的连接的HRP级联，形成可回收的双酶级联系统以检测葡萄糖。

更新日期：2018-01-10

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