DNA duplexes consisting of a 25mer together with shorter complementary sequences were studied over a range of temperature and surface binding motifs using a coarse-grained two-site nucleotide model. Results were analyzed in terms of hydrogen bonding interactions and structural characteristics and indicate that hybridization is most stable when furthest from the surface binding site. Strand elongation and straightening near the bound end are found to be correlated to duplex destabilization.

中文翻译：

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通过蒙特卡洛分子模拟研究了不同探针长度和靶链长度在DNA微阵列中的作用

使用一个粗粒度的两个位点核苷酸模型，在一定温度和表面结合基序的范围内研究了由25mer和较短互补序列组成的DNA双链体。根据氢键相互作用和结构特征对结果进行了分析，结果表明，当距离表面结合位点最远时，杂交是最稳定的。发现在结合端附近的链伸长和拉直与双链体不稳定相关。