This report outlines a highly sensitive and facile electrochemiluminescence (ECL) sensing platform based on a novel high-quantum-yield Au-nanocluster (AuNC) probe for glutathione (GSH) detection. Owing to the prominent quenching effect of GSH on the ECL of the AuNCs, the proposed ECL nanosensor showed a wide response to GSH in the ranges of 1.0 × 10^-9–1.0 × 10^-5 M and 1.0 × 10^-5–1.0 × 10^-1 M and a low detection limit of 3.2 × 10^-10 M. In addition, the proposed system exhibited good selectivity for GSH in the presence of other chemical/biological interferences. Moreover, since no further functionalization of AuNC-based sensor interface was necessary, together with the stability, high sensitivity and selectivity of the proposed nanosensor, this convenient approach was able to successfully detect GSH in both of human urine samples and blood samples with excellent recoveries, which indicated its promising application under physiological conditions. Of significant importance is that this study not only helps in gaining a better understanding of the applicability of the ECL properties of AuNCs, but also provides a new avenue for the design and development of ECL sensors based on the novel high-quantum-yield AuNC-based probe and other functional-metal-based NC probes.

本报告概述了一种基于新型高量子产率的金纳米簇（AuNC）探针的高灵敏且简便的电化学发光（ECL）传感平台，用于谷胱甘肽（GSH）检测。由于GSH对AuNCs的ECL具有显着的猝灭作用，因此所提出的ECL纳米传感器在1.0×10 ^-9 –1.0×10 ^-5  M和1.0×10 ^-5 –1.0×的范围内显示出对GSH的宽响应。10 ^-1  M和3.2×10 ^-10的低检测限 M.此外，在存在其他化学/生物干扰的情况下，拟议的系统对GSH表现出良好的选择性。此外，由于不再需要基于AuNC的传感器接口的进一步功能化，以及所提议的纳米传感器的稳定性，高灵敏度和选择性，因此这种便捷的方法能够成功地检测人尿液样品和血液样品中的GSH，回收率极高，表明其在生理条件下的应用前景广阔。至关重要的是，这项研究不仅有助于更好地了解AuNC的ECL特性的适用性，而且还为基于新型高量子产率AuNC-的ECL传感器的设计和开发提供了新途径。基探针和其他基于功能金属的NC探针。