The long exposition to reactive species results in oxidative stress which has been related with the development of cancer and other serious diseases. Olea europaea and Prunus persica seeds present a high protein content and preliminary results demonstrated their high potency to obtain bioactive peptides. The protective effect against oxidative damage exerted by peptides released from Olea europaea and Prunus persica seeds has been evaluated in this work. Seed hydrolysates showed protection against oxidation through four different mechanisms: inhibition of the formation of hydroxyl radicals, scavenging of free radicals, reduction of oxidizing compounds, and inhibition of lipid peroxidation. Moreover, seed hydrolysates also reduced the oxidative stress induced by an oxidizing agent on human cancer cells. Despite protection evaluated by individual mechanisms seemed to be significantly affected by the seed genotype, overall protection of seed hydrolysates was not so different. Seeds hydrolysates were not cytotoxic on normal cells but they demonstrated antiproliferative effect on human cancer cells (HeLa, PC-3, and HT-29). Peptides in all seed hydrolysates were sequenced by RP-HPLC-ESI-Q-TOF. Eighteen common peptides were observed among olive seed hydrolysates while a wider variability was observed among Prunus seed hydrolysates.

长时间暴露于反应性物种会导致氧化应激，这与癌症和其他严重疾病的发展有关。油橄榄和欧洲李子种子具有较高的蛋白质含量，初步结果表明它们具有很高的获得生物活性肽的能力。油橄榄和欧洲李释放的肽对氧化损伤的保护作用种子已在这项工作中进行了评估。种子水解物通过四种不同的机制显示出抗氧化的作用：抑制羟基自由基的形成，清除自由基，减少氧化性化合物和抑制脂质过氧化。此外，种子水解产物还降低了氧化剂对人癌细胞产生的氧化应激。尽管通过个体机制评估的保护似乎受种子基因型的影响很大，但种子水解产物的总体保护并没有太大不同。种子水解产物对正常细胞无细胞毒性，但对人癌细胞（HeLa，PC-3和HT-29）具有抗增殖作用。通过RP-HPLC-ESI-Q-TOF对所有种子水解物中的肽进行测序。李属种子水解产物。