Tuberculosis (TB) is still one of the important causes of death worldwide. Its detection is challenging and new tools for TB diagnostics are highly needed. In this work, we investigated a possibility of using surface plasmon resonance (SPR) phenomenon in detection of TB bacteria in sputum. For this purpose we designed a portable SPR apparatus. We compared its performance with a standard desktop SPR platform designed to work in laboratory by measuring response to Ag85 – TB secretory protein. We examined samples of suspended Mycobacterium tuberculosis cultures and sputum samples of TB patients. The device was able to detect M. tuberculosis secretory protein (Ag85) in concentration of 10 ng/ml which was comparable to the desktop SPR equipment. It has been also able to detect TB bacteria cultures in concentration of 1*10⁴ cfu/ml with no significant interfering response from two other bacteria species. Finally, detection of bacteria in sputum of confirmed tuberculosis patients has been demonstrated.

结核病（TB）仍然是世界范围内重要的死亡原因之一。它的检测具有挑战性，因此急需用于结核病诊断的新工具。在这项工作中，我们调查了使用表面等离子体共振（SPR）现象检测痰中TB细菌的可能性。为此，我们设计了一种便携式SPR设备。我们通过测量对Ag85 – TB分泌蛋白的反应，将其性能与设计用于实验室的标准台式SPR平台进行了比较。我们检查了结核分枝杆菌悬浮培养的样本和结核病患者的痰样本。该设备能够检测结核分枝杆菌浓度为10 ng / ml的分泌蛋白（Ag85），可与台式SPR设备媲美。它也能够检测浓度为1 * 10 ⁴  cfu / ml的TB细菌培养物，而没有来自其他两种细菌的​​明显干扰反应。最后，已证实在确诊的肺结核患者的痰液中检测细菌。