An electrochemiluminescence biosensing platform for Hg2+ determination based on host–guest interactions between β-cyclodextrin functionalized Pd nanoparticles and ferrocene,Analytical Methods

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An electrochemiluminescence biosensing platform for Hg2+ determination based on host–guest interactions between β-cyclodextrin functionalized Pd nanoparticles and ferrocene
Analytical Methods ( IF 2.7 ) Pub Date : 2018-01-10 00:00:00 , DOI: 10.1039/c7ay02944f
Yijie Hu _{1,

2,

3,

4} , Zhimin Liu _{1,

2,

3,

4} , Haijun Zhan _{1,

2,

3,

4} , Zhiqiang Shen _{1,

2,

3,

4}

Affiliation

A solid-state electrochemiluminescence (ECL) switch biosensor using a Ru(bpy)₃²⁺/β-cyclodextrin–Pd nanoparticles (β-CY–PdNPs)/gelatin (Gel) complex and a ferrocene-labeled DNA probe (Fer-DNA) for the detection of Hg²⁺ was successfully developed. The ECL biosensor includes an ECL substrate and an ECL signal switch. The Ru(bpy)₃²⁺/β-CY–PdNPs/Gel composite modified on a glassy carbon electrode was used as the ECL substrate, which could bring about a clear and stable ECL signal by Ru(bpy)₃²⁺. Meantime, the hairpin-like Fer-DNA probe acted as the ECL signal switch, which was designed by a molecular recognition strategy and attached to β-CY–PdNPs through host–guest interactions between β-CY and ferrocene. Our investigation indicated that, when Hg²⁺ was absent, the Fer-DNA probe retained its hairpin structure and led to an obvious quenching effect of the Ru(bpy)₃²⁺ signal. However, when the biosensor was incubated with Hg²⁺, the specific T–Hg²⁺–T interaction brought about a change of the Fer-DNA conformation, and such conformation adjustment resulted in a clear signal recovery of Ru(bpy)₃²⁺ owing to the reduced quenching effect of ferrocene. The ECL switch biosensor offered good linear responses for Hg²⁺ in the range of 0.003–600 ng mL⁻¹ with a detection limit of 0.0015 ng mL⁻¹ at the 3s_blank level.

中文翻译：

基于β-环糊精官能化的Pd纳米颗粒与二茂铁之间的主客体相互作用的 Hg ²⁺电化学发光生物传感平台

固态电化学发光（ECL）开关生物传感器，使用Ru（bpy）₃²⁺ /β-环糊精-Pd纳米颗粒（β-CY-PdNPs）/明胶（Gel）复合物和二茂铁标记的DNA探针（Fer-DNA ）用于检测Hg ^2+的成功开发。ECL生物传感器包括ECL基板和ECL信号开关。以玻碳电极修饰的Ru（bpy）₃²⁺ /β-CY-PdNPs/ Gel复合材料作为ECL基质，通过Ru（bpy）₃²⁺可以产生清晰稳定的ECL信号。。同时，发夹状的Fer-DNA探针充当ECL信号开关，它是通过分子识别策略设计的，并通过β-CY和二茂铁之间的客体相互作用与β-CY-PdNPs相连。我们的研究表明，当不存在Hg ²⁺时，Fer-DNA探针保留了其发夹结构，并导致了Ru（bpy）₃²⁺信号的明显猝灭作用。但是，当生物传感器与Hg ²⁺一起孵育时，特定的T–Hg ²⁺ –T相互作用导致Fer-DNA构象发生变化，并且这种构象调整导致Ru（bpy）₃^2的信号明显恢复。⁺由于二茂铁的淬灭作用降低。ECL开关生物传感器在0.003–600 ng mL ^-1的范围内对Hg ²⁺提供了良好的线性响应，在3s_空白水平下的检测极限为0.0015 ng mL ^-1。

更新日期：2018-01-10

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