Hydrogen-bonding-induced colorimetric detection of melamine based on the peroxidase activity of gelatin-coated cerium oxide nanospheres†,Analytical Methods

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Hydrogen-bonding-induced colorimetric detection of melamine based on the peroxidase activity of gelatin-coated cerium oxide nanospheres†
Analytical Methods ( IF 2.7 ) Pub Date : 2018-01-10 00:00:00 , DOI: 10.1039/c7ay02296d
Xiaoyong Jin _{1,

2,

3,

4,

5} , Wenqing Yin _{1,

2,

3,

4,

5} , Gang Ni _{1,

2,

3,

4,

5} , Juan Peng _{1,

2,

3,

4,

5}

Affiliation

Herein, we developed a simple and rapid colorimetric assay for the detection of melamine using gelatin-coated cerium oxide (Gel-CeO₂) nanospheres as peroxidase mimics. Highly monodisperse Gel-CeO₂ nanospheres were synthesized through a microwave-assisted hydrothermal process. The Gel-CeO₂ nanospheres showed excellent peroxidase activity, which can catalyze the oxidation of ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) by H₂O₂, resulting in the formation of blue oxidation products. In the presence of melamine, H₂O₂ will react with melamine through strong hydrogen-bonding. With the consumption of H₂O₂, the catalytic reaction was interrupted and the blue ABTS oxidation product solution turned pale. There was a linear relationship between the absorbance intensity of the ABTS oxidation product and the logarithm values of melamine concentrations ranging from 50 nM to 5.0 mM. Moreover, the detection limit (S/N = 3) was 5.5 nM, which is far below the regulatory level. This method was simple, rapid, sensitive and reliable, suggesting the promising practical usage of this sensing system. Finally, this method was applied to melamine detection in milk and milk powder.

中文翻译：

基于明胶包覆的氧化铈纳米球的过氧化物酶活性，氢键诱导的三聚氰胺比色检测†

在这里，我们开发了一种简单而快速的比色测定法，使用明胶包被的氧化铈（Gel-CeO ₂）纳米球作为过氧化物酶模拟物来检测三聚氰胺。通过微波辅助水热法合成了高度单分散的Gel-CeO ₂纳米球。Gel-CeO ₂纳米球表现出优异的过氧化物酶活性，可以催化AB 2（2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）二铵盐）被H ₂ O ₂氧化，从而形成蓝色氧化产物。在三聚氰胺的存在下，H ₂ O ₂将通过强氢键与三聚氰胺反应。随着H ₂ O的消耗_{如图2所示}，催化反应中断并且蓝色ABTS氧化产物溶液变浅。ABTS氧化产物的吸收强度与三聚氰胺浓度的对数值在50 nM至5.0 mM之间存在线性关系。此外，检出限（S / N = 3）为5.5 nM，远低于法规水平。该方法简单，快速，灵敏且可靠，表明该传感系统的实际应用前景广阔。最后，该方法用于牛奶和奶粉中三聚氰胺的检测。

更新日期：2018-01-10

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