Multiplexed detection of nucleic acids using 19F NMR chemical shift changes based on DNA photo-cross-linking of 3-vinylcarbazole derivatives†,Organic & Biomolecular Chemistry

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Multiplexed detection of nucleic acids using 19F NMR chemical shift changes based on DNA photo-cross-linking of 3-vinylcarbazole derivatives†
Organic & Biomolecular Chemistry ( IF 2.9 ) Pub Date : 2018-01-10 00:00:00 , DOI: 10.1039/c7ob03008h
Shigetaka Nakamura _{1,

2,

3,

4} , Yasuharu Takashima _{1,

2,

3,

4} , Kenzo Fujimoto _{1,

2,

3,

4}

Affiliation

The detection methodology for nucleic acids is a useful tool for the analysis of biological systems and diagnosis of diseases. We demonstrated the feasibility of the detection of any nucleic acids based on large chemical shifts via ultrafast DNA photo-cross-linking and the effects of substitution by 3-vinylcarbazole derivatives. These chemical shifts enable the sequence-specific detection of any strand using hybridization chain reaction.

中文翻译：

使用基于3乙烯基咔唑衍生物的DNA光交联的¹⁹ F NMR化学位移变化对核酸进行多重检测†

核酸的检测方法学是用于分析生物系统和诊断疾病的有用工具。我们证明了通过超快速DNA光交联检测基于大化学位移的任何核酸的可行性以及被3-乙烯基咔唑衍生物取代的效果。这些化学位移使得可以使用杂交链反应对任何链进行序列特异性检测。

更新日期：2018-01-10

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