C-phycocyanin (C-PC), a type of functional food colourant, plays a significant role in regulating inflammatory responses. However, its underlying anti-inflammatory mechanism remains unknown. Previously, we have established a ³⁵S in vivo/vitro labelling analysis for dynamic proteomic (SiLAD) technology. Unlike traditional proteomic methods, SiLAD exclusively detects synthesis rates instead of accumulated amounts through ³⁵S pulse labelling of newly synthesized proteins, providing a high sensitive way for analysing protein alterations. In this work, we applied SiLAD to explore the anti-inflammatory mechanism of C-PC. We found that the protein synthesis rates were specifically detected and visualised. Strikingly, network analysis suggested programmed cell death 5 protein (PDCD5) was involved in C-PC mediated anti-inflammatory process. Phenotyping experiments demonstrated that C-PC could reduce inflammation through down-regulating PDCD5-NF-κB signaling. These results provided important information for the regulation of C-PC, and offered a novel perspective for regulatory mechanism of functional food.

C-藻蓝蛋白（C-PC）是一种功能性食品色素，在调节炎症反应中起着重要作用。然而，其潜在的抗炎机制仍然未知。以前，我们已经建立了用于动态蛋白质组学（SiLAD）技术的³⁵ S体内/体外标记分析。与传统的蛋白质组学方法不同，SiLAD专门检测合成速率，而不是通过³⁵新合成蛋白质的S脉冲标记，为分析蛋白质变化提供了一种高灵敏度的方法。在这项工作中，我们应用SiLAD来探索C-PC的抗炎机制。我们发现蛋白质的合成速率是专门检测和可视化的。令人惊讶的是，网络分析表明程序性细胞死亡5蛋白（PDCD5）参与了C-PC介导的抗炎过程。表型分析实验表明，C-PC可通过下调PDCD5-NF-κB信号传导来减轻炎症。这些结果为C-PC的调控提供了重要的信息，为功能性食品的调控机制提供了新的视角。