Antigen‐Directed Fabrication of a Multifunctional Nanovaccine with Ultrahigh Antigen Loading Efficiency for Tumor Photothermal‐Immunotherapy,Advanced Materials

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Antigen‐Directed Fabrication of a Multifunctional Nanovaccine with Ultrahigh Antigen Loading Efficiency for Tumor Photothermal‐Immunotherapy
Advanced Materials ( IF 27.4 ) Pub Date : 2018-01-10 , DOI: 10.1002/adma.201704408
Jinbin Pan ₁ , Yaqiong Wang ₁ , Cai Zhang ₂ , Xiaoyi Wang ₃ , Haoyu Wang ₁ , Jiaojiao Wang ₄ , Yizhong Yuan ₄ , Xu Wang ₅ , Xuejun Zhang ₄ , Chunshui Yu ₁ , Shao-Kai Sun ₄ , Xiu-Ping Yan _{6,

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Affiliation

Current antigen‐encapsulated multifunctional nanovaccines for oncotherapy suffer from limited antigen loading efficiency, low yield, tedious manufacture, and systemic toxicity. Here, an antigen‐directed strategy for the fabrication of multifunctional nanovaccine with ultrahigh antigen loading efficiency in a facile way for tumor photothermal‐immunotherapy is shown. As a proof of concept, a model antigen ovalbumin (OVA) is used as a natural carrier to load a representative theranostic agent indocyanine green (ICG). Mixing OVA and ICG in aqueous solution gives the simplest multifunctional nanovaccine so far. The nanovaccine owns antigen loading efficiency of 80.8%, high yield of >90%, intense near‐infrared absorption and fluorescence, excellent reproducibility, good aqueous solubility and stability, and favorable biocompatibility. These merits not only guarantee sensitive labeling/tracking and efficient stimulation of dendritic cells, but also reliable imaging‐guided photothermal‐immunotherapy of tumors and tumor prevention. The proposed strategy provides a facile and robust method for large‐scale and reproducible fabrication of multifunctional nanovaccines with ultrahigh antigen loading efficiency for tumor therapy.

中文翻译：

具有超高抗原装载效率的多功能纳米疫苗的抗原导向制备，用于肿瘤光热免疫治疗。

当前用于肿瘤治疗的抗原封装多功能纳米疫苗的抗原负载效率有限，产量低，生产繁琐且具有全身毒性。在这里，展示了一种抗原导向的策略，该方法以一种简便的方法制造具有超高抗原装载效率的多功能纳米疫苗，用于肿瘤光热免疫疗法。作为概念的证明，模型抗原卵清蛋白（OVA）被用作天然载体，以加载代表性的治疗病原体吲哚菁绿（ICG）。迄今为止，在水溶液中混合OVA和ICG可得到最简单的多功能纳米疫苗。纳米疫苗具有80.8％的抗原负载效率，> 90％的高收率，强烈的近红外吸收和荧光，出色的重现性，良好的水溶性和稳定性以及良好的生物相容性。这些优点不仅保证了对树突状细胞的敏感标记/跟踪和有效刺激，而且还保证了可靠的肿瘤成像引导光热免疫疗法和肿瘤预防。所提出的策略为大规模和可重现的多功能纳米疫苗的制备提供了一种简便而鲁棒的方法，具有超高的抗原加载效率，可用于肿瘤治疗。

更新日期：2018-01-10

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