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Electrochemical detection of microRNAs by graphene oxide modified disposable graphite electrodes
Journal of Electroanalytical Chemistry ( IF 4.1 ) Pub Date : 2018-02-01 , DOI: 10.1016/j.jelechem.2018.01.015
Ece Eksin , Arzum Erdem

Abstract Graphene oxide (GO) modified disposable pencil graphite electrodes (PGEs) were developed for the first time in the present study for miRNA detection by measuring the guanine oxidation signal. GO modified PGEs were used as solid phase to immobilize amino linked DNA probe and then the complementary target RNA sequence (miRNA-34a) was recognized. The electrochemical behaviour of the GO modified electrodes was investigated by using electrochemical impedance spectroscopy (EIS) and microscopic characterization was performed by scanning electron microscopy (SEM). The optimization studies and detection of hybridization between miRNA-34a target and its complementary DNA probe was performed by differential pulse voltammetry (DPV). The selectivity of our assay was tested in the presence of non-complementary miRNA sequence. A good discrimination could be achieved compared to the results obtained with the full match hybridization of probe with its RNA target miRNA-34a target against other miRNA sequences; miRNA-16 and miRNA-155.

中文翻译:

氧化石墨烯修饰的一次性石墨电极电化学检测微小RNA

摘要 本研究首次开发了氧化石墨烯 (GO) 修饰的一次性铅笔石墨电极 (PGE),通过测量鸟嘌呤氧化信号进行 miRNA 检测。GO修饰的PGEs用作固相固定氨基连接的DNA探针,然后识别互补的靶RNA序列(miRNA-34a)。通过使用电化学阻抗谱 (EIS) 研究了 GO 修饰电极的电化学行为,并通过扫描电子显微镜 (SEM) 进行了微观表征。miRNA-34a 靶标与其互补 DNA 探针之间杂交的优化研究和检测是通过差分脉冲伏安法 (DPV) 进行的。在存在非互补 miRNA 序列的情况下测试了我们测定的选择性。与探针与其 RNA 靶标 miRNA-34a 靶标与其他 miRNA 序列的完全匹配杂交所获得的结果相比,可以实现良好的区分;miRNA-16 和 miRNA-155。
更新日期:2018-02-01
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