Ocean acidification (OA) may affect sensitivity of marine organisms to metal pollution modulating chemical bioavailability, bioaccumulation and biological responsiveness of several cellular pathways. In this study, the smooth scallop Flexopecten glaber was exposed to various combinations of reduced pH (pH/pCO₂ 7.4/∼3000 μatm) and Cd (20 μg/L). The analyses on cadmium uptake were integrated with those of a wide battery of biomarkers including metallothioneins, single antioxidant defenses and total oxyradical scavenging capacity in digestive gland and gills, lysosomal membrane stability and onset of genotoxic damage in haemocytes. Reduced pH slightly increased concentration of Cd in scallop tissues, but no effects were measured in terms of metallothioneins. Induction of some antioxidants by Cd and/or low pH in the digestive gland was not reflected in variations of the total oxyradical scavenging capacity, while the investigated stressors caused a certain inhibition of antioxidants and reduction of the scavenging capacity toward peroxyl radical in the gills. Lysosomal membrane stability and onset of genotoxic damages showed high sensitivity with possible synergistic effects of the investigated factors. The overall results suggest that indirect effects of ocean acidification on metal accumulation and toxicity are tissue-specific and modulate oxidative balance through different mechanisms.

海洋酸化（OA）可能会影响海洋生物对金属污染的敏感性，从而调节几种细胞途径的化学生物利用度，生物蓄积性和生物响应性。在这项研究中，光滑的扇贝Flexopecten glaber暴露于各种降低的pH值（pH / p CO ₂7.4 /〜3000μatm）和Cd（20μg/ L）。对镉吸收的分析与包括生物硫蛋白，单一抗氧化剂防御和消化腺和and中总氧自由基清除能力，溶酶体膜稳定性和血细胞遗传毒性损害的发作在内的一系列生物标志物的分析相结合。降低的pH值会稍微增加扇贝组织中Cd的浓度，但没有检测到金属硫蛋白的影响。镉和/或低pH值在消化腺中诱导的某些抗氧化剂未反映在总氧自由基清除能力的变化中，而所研究的应激源对a中的过氧化物自由基产生了一定程度的抗氧化剂抑制作用和清除能力。溶酶体膜的稳定性和遗传毒性损伤的发作显示出高的敏感性，可能与所研究因素的协同作用有关。总体结果表明，海洋酸化对金属积累和毒性的间接影响是组织特异性的，并通过不同的机制调节氧化平衡。