In the present study, we are reporting the synthesis of a triazoles incorporated fluorescent hexahydroquinoline appended calix[4]arene 7 and its highly selective optical recognition ability towards Hg²⁺. The optical sensor 7 was synthesized via two different synthetic pathways and unambiguously characterized by mass spectrometry and NMR spectroscopy. The stoichiometric ratio between 7 and Hg²⁺ was determined as 1:1 based on Job’s plot and ESI-MS analysis. The chemosensor 7 selectively recognized Hg²⁺ in the presence of competitive cations such as Cu²⁺, Cu¹⁺, Co³⁺, Ni²⁺, Ag¹⁺, Fe²⁺, Fe³⁺, Cr³⁺, Pb²⁺, Cd²⁺, Zn²⁺, Sn²⁺, Ti⁴⁺, Sb³⁺, In³⁺, Ba²⁺, Ca²⁺, and K¹⁺. The limit of detection (LOD) was established as 0.5 µM on spectrofluorimetric analysis. According to world health organization (WHO) in guidelines for drinking-water quality, the mercury level in natural occurring ground and surface water is about ~ 0.5 µM (µg/L). The supra molecule 7 with LOD (0.5 µM) could potentially serve effective receptors for Hg²⁺ cation. The probe 7 was applied effectively for detection of Hg²⁺ in real water samples (i.e. tap and deionized water), spiked with Hg²⁺ (10 µM) solution. Moreover, the compound 7 showed non-toxicity during cytotoxic assay against human B cells as they retain their morphology. The supramolecule 7 in living cells showed selectivity towards Hg²⁺ in cytotoxic assay with T lymphocytes, evident by morphological changes observed via AFM analysis.

在本研究中，我们报告了结合有荧光六氢喹啉的三唑并附有杯[ 4 ]芳烃7的合成及其对Hg ^2+的高度选择性光学识别能力。光学传感器7是通过两种不同的合成途径合成的，并且通过质谱法和NMR光谱法明确地表征。根据Job's图和ESI-MS分析，确定7与Hg ²⁺之间的化学计量比为1：1。化学传感器7在竞争性阳离子（例如Cu 2 ⁺，Cu ^1+）存在下选择性地识别Hg ²⁺，Co ³⁺，Ni 2 ⁺，Ag ¹⁺，Fe 2 ⁺，Fe ³⁺，Cr ³⁺，Pb 2 ⁺，Cd 2 ⁺，Zn 2 ⁺，Sn 2 ⁺，Ti ⁴⁺，Sb ³⁺，In ³⁺，Ba 2 ⁺，Ca ²⁺和K ¹⁺。检出限（LOD）的极限被确定为0.5 μ对荧光光度法分析微米。根据世界卫生组织（WHO）的饮用水水质准则，天然地下水和地表水中的汞含量约为〜0.5 µM（µ g / L）。在上文分子7与LOD（0.5 μ M）可以潜在地用作有效的受体对Hg ²⁺阳离子。探头7被检测汞有效地应用于²⁺实际水样中（即自来水和去离子水）中，用汞掺入²⁺（10 μ M）溶液。而且，化合物7在针对人B细胞的细胞毒性测定过程中显示出无毒性，因为它们保留了其形态。活细胞中的超分子7在T淋巴细胞的细胞毒性测定中显示出对Hg ^2+的选择性，这在观察到的形态学变化中很明显通过AFM分析。