A series of novel coumarin-theophylline hybrids were synthesized and examined for their anti-tubercular activity in vitro against Mycobacterium tuberculosis H₃₇Rv, anti-microbial activity in vitro against gram-positive bacteria (Staphylococcus aureus) and gram-negative bacterias (Escherichia coli, Salmonella typhi) as well as fungi (Candida albicans). The compound (3a) has shown excellent anti-tubercular activity with MIC of 0.12 μg/mL. Electron donating compounds (3a, 3f) have displayed significant anti-microbial activity. The compounds have also been precisely elucidated using single crystal X-ray diffraction techniques. Molecular docking study has been performed against 4DQU enzyme of Mycobacterium tuberculosis showed good binding interactions and is in agreement with the in vitro results.

合成了一系列新型香豆素-茶碱杂种，并测试了它们在体外对结核分枝杆菌H ₃₇ Rv的抗结核活性，在体外对革兰氏阳性菌（金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌）的抗微生物活性。，沙门氏菌（Salmonella typhi）以及真菌（白色念珠菌（Candida albicans））。化合物（3a）显示出优异的抗结核活性，MIC为0.12μg/ mL。给电子化合物（3a，3f）表现出显着的抗微生物活性。还使用单晶X射线衍射技术精确阐明了这些化合物。已经针对结核分枝杆菌的4DQU酶进行了分子对接研究，显示出良好的结合相互作用，并且与体外结果相符。