Recent research studies have shown that the intestinal flora are related to the occurrence and progress of breast cancer. This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers. Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively. Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition. The contents of D-lactic acid (D-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA. Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting. Our results suggested that the intestinal wall of the model group was damaged. However, after fucoidan intervention, the villi were gradually restored. ELISA showed that the levels of plasma endotoxin, D-LA and DAO decreased in the F1 and F2 groups compared to those in the model group. Fucoidan treatment also increased the expressions of ZO-1, occludin, claudin-1 and claudin-8. Furthermore, the expression levels of phosphorylated p38 MAPK and ERK1/2 were upregulated in fucoidan treatment groups. The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio. In conclusion, the supplement of fucoidan could improve the fecal microbiota composition and repair the intestinal barrier function. The study suggested the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer.

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岩藻依聚糖对乳腺癌大鼠肠道菌群和肠屏障功能的影响

最近的研究表明，肠道菌群与乳腺癌的发生和发展有关。这项研究调查了岩藻依聚糖对7,12-二甲基苯并[ a ]蒽（DMBA）诱导的乳腺癌大鼠肠道菌群和肠屏障功能的影响。将60只Sprague-Dawley雌性大鼠随机分为对照组，模型组以及F1和F2组，分别以每公斤体重200和400 mg体重的岩藻依聚糖喂养。进行了肠道组织病理学分析，并使用16S rDNA高通量测序对肠道菌群组成进行了概述。的内容d乳酸（d-LA），血浆中的二胺氧化酶（DAO）和内毒素。使用蛋白质印迹法检测紧密连接（TJ）蛋白，磷酸化的p38 MAPK和ERK1 / 2的表达水平。我们的结果表明该模型组的肠壁被破坏。但是，在岩藻依聚糖干预后，绒毛逐渐恢复。ELISA法显示血浆内毒素D与模型组相比，F1和F2组的-LA和DAO降低。Fucoidan处理还增加了ZO-1，occludin，claudin-1和claudin-8的表达。此外，岩藻依聚糖治疗组的磷酸化p38 MAPK和ERK1 / 2的表达水平上调。16S rDNA高通量测序的结果表明，岩藻依聚糖增加了肠道菌群的多样性，并诱导了微生物组成的变化，同时提高了拟杆菌/拟杆菌门的比率。总之，岩藻依聚糖的补充可以改善粪便微生物群的组成并修复肠屏障功能。该研究建议使用岩藻依聚糖作为肠道菌群调节剂来潜在地预防乳腺癌。