A newly designed quantum dots and manganese dioxide nanosheets nanocomposite-based fluorescence lateral flow biosensor was developed for rapid detection of glutathione (GSH). In this biosensing system, nanocomposites of biotinylated quantum dots (Biotin-QDs) and MnO₂ nanosheets were first fixed on the conjugation pad of the test strip. The addition of GSH could trigger the decomposition of nanocomposites by digesting MnO₂ nanosheets and consequently release Biotin-QDs. Under the capillary effect, the free Biotin-QDs would continue to move forward and be captured by streptavidin (SA) on the test line due to the specific binding between biotin and SA. Thereafter, an obvious fluorescent enhancement can be observed on the test line with a portable strip reader as well as naked eyes under irradiation by a 365 nm handheld ultraviolet lamps, which was used successfully for the quantitative detection of GSH with a short assay time of 20 min. Furthermore, this biosensor was utilized for GSH detection in diluted HeLa cells extracts (1%) samples with satisfactory results, which can meet low concentration detection for GSH with a good linear relationship in the range from 0.05 to 1.2 mM. Holding the advantages such as simple, convenient, rapid and cost effective, this developed fluorescence lateral flow biosensor has a great potential to be used for GSH detection in point of care diagnosis.

一种新型设计的基于量子点和二氧化锰纳米片的纳米复合荧光侧流生物传感器用于谷胱甘肽（GSH）的快速检测。在这种生物传感系统中，首先将生物素化量子点（Biotin-QDs）和MnO ₂纳米片的纳米复合材料固定在测试条的偶联垫上。谷胱甘肽的添加可通过消化MnO ₂引发纳米复合材料的分解纳米片并因此释放生物素-量子点。在毛细作用下，由于生物素和SA之间的特异性结合，游离的生物素-QD将继续向前移动并被抗生蛋白链菌素（SA）捕获在测试线上。此后，在便携式365手持式紫外线灯照射下，使用便携式带状读取器和肉眼可以在测试线上观察到明显的荧光增强，该荧光灯已成功用于定量检测GSH，检测时间短至20分钟 此外，该生物传感器用于稀释的HeLa细胞提取物（1％）样品中的GSH检测，结果令人满意，可以满足低浓度的GSH检测，线性关系良好，范围为0.05至1.2 mM。拥有简单，方便，快速，经济高效的优势，