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Using Chlorella vulgaris to treat toxic excess sludge extract, and identification of its response mechanism by proteomics approach
Bioresource Technology ( IF 9.7 ) Pub Date : 2018-01-09 , DOI: 10.1016/j.biortech.2018.01.039
Lu Wang , Hualin Wang , Xiurong Chen , Yan Xu , Tianjun Zhou , Xiaoxiao Wang , Qian Lu , Roger Ruan

was cultivated in varying proportions of toxic sludge extracts obtained from a sequencing batch reactor for treating synthetic wastewater containing chlorophenols. could reduce the ecotoxicity from sludge extracts, and a positive correlation was noted between ecotoxicity removal and total organic carbon removal. In terms of cell density, the optimal proportion of sludge extracts required for the cultivation of was lower than 50%. The correlation between protein content in per 10 algae and inhibition extent of ecotoxicity of the 5 groups on the day of inoculation (0.9182, < .05) indicated a positive relationship between algal protein secretion and ecotoxicity. According to the protein expression and differential protein expression analysis, we concluded that produced proteins that involved in the stress response/redox system and energy metabolism/biosynthesis to respond to the toxic environment and some other proteins related to mixotrophic metabolism.

中文翻译:


利用普通小球藻处理有毒剩余污泥提取物并通过蛋白质组学方法鉴定其响应机制



是用不同比例的有毒污泥提取物进行培养的,这些污泥提取物是从序批式反应器中获得的,用于处理含有氯酚的合成废水。可以降低污泥提取物的生态毒性,并且生态毒性去除与总有机碳去除之间存在正相关关系。从细胞密度来看,培养所需污泥提取物的最佳比例低于50%。接种当日5组每10只藻中蛋白质含量与生态毒性抑制程度的相关性(0.9182,<.05)表明藻类蛋白质分泌与生态毒性之间呈正相关。根据蛋白质表达和差异蛋白表达分析,我们得出结论,产生了参与应激反应/氧化还原系统和能量代谢/生物合成以响应有毒环境的蛋白质以及一些与混合营养代谢相关的其他蛋白质。
更新日期:2018-01-09
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