The overuse of antibiotics has posed a propagation of antibiotic resistance genes (ARGs) in aquaculture systems. This study firstly explored the ARGs profiles of the typical mariculture farms including conventional and recirculating systems using metagenomics approach. Fifty ARGs subtypes belonging to 21 ARGs types were identified, showing the wide-spectrum profiles of ARGs in the coastal industrial mariculture systems. ARGs with multiple antibiotics resistance have emerged in the mariculure systems. The co-occurrence pattern between ARGs and microbial taxa showed that Proteobacteria and Bacteroidetes were potential dominant hosts of ARGs in the industrial mariculture systems. Typical nitrifying bacteria such as Nitrospinae in mariculture systems also carried with some resistance genes. Relative abundance of ARGs in fish ponds and wastewater treatment units was relatively high. The investigation showed that industrial mariculture systems were important ARGs reservoirs in coastal area, indicating the critical role of recirculating systems in the terms of ARGs pollution control.

中文翻译：

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沿海工业化海水养殖系统抗生素抗性基因的宏基因组分析


抗生素的过度使用导致了水产养殖系统中抗生素抗性基因（ARG）的传播。本研究首先利用宏基因组学方法探讨了典型海水养殖场（包括常规系统和再循环系统）的 ARGs 概况。鉴定出属于 21 个 ARG 类型的 50 个 ARG 亚型，显示了沿海工业化海水养殖系统中 ARG 的广谱特征。具有多种抗生素耐药性的 ARG 已经出现在海藻系统中。 ARGs 和微生物类群之间的共现模式表明变形菌门和拟杆菌门是工业化海水养殖系统中 ARGs 的潜在优势宿主。海水养殖系统中的典型硝化细菌如Nitrospinae也携带一些抗性基因。鱼塘和废水处理装置中ARGs的相对丰度较高。调查表明，工业化海水养殖系统是沿海地区重要的ARGs库，表明再循环系统在ARGs污染控制中的关键作用。