Serine Integrases: Advancing Synthetic Biology,ACS Synthetic Biology

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Serine Integrases: Advancing Synthetic Biology
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2018-01-30 00:00:00 , DOI: 10.1021/acssynbio.7b00308
Christine A. Merrick ₁ , Jia Zhao ₂ , Susan J. Rosser ₁

Affiliation

Serine integrases catalyze precise rearrangement of DNA through site-specific recombination of small sequences of DNA called attachment (att) sites. Unlike other site-specific recombinases, the recombination reaction driven by serine integrases is highly directional and can only be reversed in the presence of an accessory protein called a recombination directionality factor (RDF). The ability to control reaction directionality has led to the development of serine integrases as tools for controlled rearrangement and modification of DNA in synthetic biology, gene therapy, and biotechnology. This review discusses recent advances in serine integrase technologies focusing on their applications in genome engineering, DNA assembly, and logic and data storage devices.

中文翻译：

丝氨酸整合：推进合成生物学。

丝氨酸整合可通过称为附着（att）位点的小序列DNA的位点特异性重组来催化DNA的精确重排。与其他位点特异性重组酶不同，丝氨酸整合驱动的重组反应具有高度的方向性，只能在存在称为重组方向性因子（RDF）的辅助蛋白的情况下逆转。控制反应方向性的能力导致了丝氨酸整合体的发展，作为在合成生物学，基因治疗和生物技术中控制DNA重排和修饰的工具。这篇评论讨论了丝氨酸整合酶技术的最新进展，重点是它们在基因组工程，DNA组装以及逻辑和数据存储设备中的应用。

更新日期：2018-01-30

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