We developed a three-dimensional biointerface of graphene-based electrical impedance sensor for metastatic cancer diagnosis at single-cell resolution. Compared with traditional impedance sensor with two-dimensional interface, the graphene biointerface mimiced the topography and somatotype features of cancer cells, achieving more comprehensive and thorough single cell signals in the three-dimensional space. At the nodes of physiological behavior change of single cell, namely cell capture, adhesion, migration and proliferation, the collected electrical signals from graphene biointerface were about two times stronger than those from the two-dimensional gold interface due to the substantial increase in contact area and significant improvement of topographical interaction between cells and graphene electrode. Simultaneous CCD recording and electrical signal extraction from the entrapped single cell on the graphene biointerface enabled to investigate multidimensional cell-electrode interactions and predict cancerous stage and pathology.

我们开发了基于石墨烯的电阻抗传感器的三维生物接口，可用于单细胞分辨率的转移性癌症诊断。与具有二维界面的传统阻抗传感器相比，石墨烯生物界面模仿了癌细胞的形貌和体型特征，在三维空间中获得了更全面，更彻底的单细胞信号。在单细胞的生理行为变化的节点上，即细胞捕获，粘附，迁移和增殖，由于接触面积的大幅增加，从石墨烯生物界面收集的电信号比从二维金界面收集的电信号强约两倍。并显着改善了细胞与石墨烯电极之间的形貌相互作用。