The residue depletion of sulfamethazine (SMZ) was evaluated in tilapia (Oreochromis niloticus) after 11 days of administration of medicated feed containing SMZ, at the dose of 422 mg/kg body weight (bw). The determination of SMZ in feed and tilapia fillet was carried out using the QuEChERS approach for sample preparation, and high performance liquid chromatography with diode array detector (HPLC-DAD) and ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QToF-MS) for quantitation, respectively. Both methods were validated based on international and Brazilian guidelines and shown to be suitable for the intended purposes. The withdrawal period to reach the maximum residue level (MRL) of 100 μg/kg, according to the European Union (EU) legislative framework to all substances belonging to the sulfonamide (SA) group (EU, 2010), was 10 days (260 °C-day). After treatment, the maximum level of SMZ accumulation in the tilapia muscle was 1.6 mg/kg. SMZ was shown to be quickly excreted by tilapia. Thus, considering the acceptable daily intake of SMZ established by the Codex Commission (0–0.05 mg/kg bw), and a factor of 5 times the upper amount of fish consumption in Brazil (38 kg/year), this study showed that there is a low risk of adverse effects to consumers. This study offers subsidies not only for the establishment of public policies with regard to the use of veterinary drugs currently not allowed in a country by their legal legislative framework for fish farming, but also to fish producers for the proper handling to ensure safe fish fillets.

在罗非鱼（Oreochromis niloticus）中评估了磺胺二甲嘧啶（SMZ）的残留消耗）在服用含SMZ的含药饲料11天后，剂量为422 mg / kg体重（bw）。饲料和罗非鱼片中SMZ的测定采用QuEChERS方法进行样品制备，并采用二极管阵列检测器（HPLC-DAD）和高效液相色谱-四极杆飞行时间质谱进行高效液相色谱分析（UPLC-QToF-MS）分别进行定量。两种方法均已根据国际和巴西指南进行了验证，并显示适用于预期目的。根据欧盟（EU）立法框架，对属于磺酰胺类（SA）组的所有物质的最大撤回水平（MRL）为100μg/ kg的撤回期（欧盟，2010年）为10天（260） °C天）。治疗后，罗非鱼肌肉中SMZ积累的最大水平为1.6 mg / kg。已显示SMZ被罗非鱼迅速排泄。因此，考虑到法典委员会确定的SMZ的每日可接受摄入量（0-0.05 mg / kg bw），是巴西鱼类消费量上限（38 kg /年）的5倍，该研究表明对消费者产生不良影响的风险很小。这项研究不仅为制定有关使用毒品的公共立法制定政策提供了补贴，目前该国在其法律法规框架下还不允许在鱼类养殖中使用兽药，而且还向鱼类生产者提供了适当处理以确保鱼片安全的补贴。考虑到法典委员会确定的SMZ可接受的每日摄入量（0-0.05 mg / kg bw），是巴西最高鱼类消费量（38 kg /年）的5倍，该研究表明对消费者不利影响的风险低。这项研究不仅为制定有关使用毒品的公共立法制定政策提供了补贴，目前该国在其法律法规框架下还不允许在鱼类养殖中使用兽药，而且还向鱼类生产者提供了适当处理以确保鱼片安全的补贴。考虑到法典委员会确定的SMZ可接受的每日摄入量（0-0.05 mg / kg bw），是巴西最高鱼类消费量（38 kg /年）的5倍，该研究表明对消费者不利影响的风险低。这项研究不仅为制定有关使用毒品的公共立法制定政策提供了补贴，目前该国在其法律法规框架下还不允许在鱼类养殖中使用兽药，而且还向鱼类生产者提供了适当处理以确保鱼片安全的补贴。