Programmable Nucleic Acid Nanoswitches for the Rapid, Single-Step Detection of Antibodies in Bodily Fluids,Journal of the American Chemical Society

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Programmable Nucleic Acid Nanoswitches for the Rapid, Single-Step Detection of Antibodies in Bodily Fluids
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2018-01-09 , DOI: 10.1021/jacs.7b09347
Alessandro Porchetta ₁ , Rudy Ippodrino ₂ , Bruna Marini ₂ , Arnaldo Caruso ₃ , Francesca Caccuri ₃ , Francesco Ricci ₁

Affiliation

Antibody detection plays a pivotal role in the diagnosis of pathogens and monitoring the success of vaccine immunization. However, current serology techniques require multiple, time-consuming washing and incubation steps, which limit their applicability in point-of-care (POC) diagnostics and high-throughput assays. We developed here a nucleic acid nanoswitch platform able to instantaneously measure immunoglobulins of type G and E (IgG and IgE) levels directly in blood serum and other bodily fluids. The system couples the advantages of target-binding induced colocalization and nucleic acid conformational-change nanoswitches. Due to the modular nature of the recognition platform, the method can potentially be applied to the detection of any antibody for which an antigen can be conjugated to a nucleic acid strand. In this work we show the sensitive, fast and cost-effective detection of four different antibodies and demonstrate the possible use of this approach for the monitoring of antibody levels in HIV+ patients immunized with AT20 therapeutic vaccine.

中文翻译：

用于快速单步检测体液中抗体的可编程核酸纳米开关

抗体检测在病原体的诊断和疫苗免疫接种的成功监测中起着举足轻重的作用。然而，当前的血清学技术需要多个耗时的洗涤和孵育步骤，这限制了它们在即时 (POC) 诊断和高通量检测中的适用性。我们在这里开发了一种核酸纳米开关平台，能够即时测量血清和其他体液中 G 型和 E 型（IgG 和 IgE）水平的免疫球蛋白。该系统结合了目标结合诱导的共定位和核酸构象变化纳米开关的优点。由于识别平台的模块化特性，该方法可潜在地应用于检测任何抗原可与核酸链缀合的抗体。在这项工作中，我们展示了敏感的，

更新日期：2018-01-09

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