The aim of this study was to investigate the anti-fatigue activity of polysaccharide fractions from Abelmoschus esculentus (L.) Moench (AE) in mice. After crude polysaccharide (CAEP) was extracted from AE and purified by DEAE cellulose-52 column, two polysaccharide fractions (AEP-1 and AEP-2) were obtained. The structural analysis suggested that AEP-1 and AEP-2 were a RG-I polysaccharide and an AG-II polysaccharide, respectively. According to the results of the weight-loaded swimming test, compared with the negative control group, the CAEP, AEP-1 and AEP-2 treatment groups could prolong the swimming time, decrease serum urea nitrogen (SUN) and blood lactic acid (BLA), and increase hepatic glycogen (HG) and muscle glycogen (MG), which indicated that okra polysaccharides have an effective anti-fatigue activity. Furthermore, our study exhibited the anti-fatigue mechanism of okra polysaccharide was correlated with retarding the accumulation of creatine kinase (CK) and lactate dehydrogenase (LDH) in serum, and enhancing succinate dehydrogenase (SDH), adenosine triphosphate (ATP) and adenosine triphosphatase (ATPase) levels. In addition, the anti-fatigue activity of AEP-1 was stronger than that of AEP-2, and significantly better than that of CAEP. Therefore, AEP-1 and AEP-2 may be the main active anti-fatigue functional substances of AE.

中文翻译：

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秋葵（Abelmoschus esculentus（L.）Moench） 多糖的纯化，表征和抗疲劳活性

这项研究的目的是研究来自Abelmoschus esculentus（L.）Moench的多糖组分的抗疲劳活性（AE）在小鼠中。从AE中提取粗多糖（CAEP）并通过DEAE纤维素-52柱纯化后，得到两个多糖级分（AEP-1和AEP-2）。结构分析表明，AEP-1和AEP-2分别是RG-1多糖和AG-II多糖。根据负重游泳试验的结果，与阴性对照组相比，CAEP，AEP-1和AEP-2治疗组可以延长游泳时间，减少血清尿素氮（SUN）和血乳酸（BLA） ），并增加肝糖原（HG）和肌肉糖原（MG），这表明秋葵多糖具有有效的抗疲劳活性。此外，我们的研究表明，秋葵多糖的抗疲劳机制与延迟血清中肌酸激酶（CK）和乳酸脱氢酶（LDH）的积累，增强琥珀酸脱氢酶（SDH），三磷酸腺苷（ATP）和腺苷三磷酸酶（ATPase）有关。 ）级别。另外，AEP-1的抗疲劳活性强于AEP-2，并且明显优于CAEP。因此，AEP-1和AEP-2可能是AE的主要活性抗疲劳功能物质。