We developed capillary zone electrophoresis (CZE) with indirect UV detection for the determination of fluoride (F⁻) in seawater using transient isotachophoresis (tITP) as an on-line concentration procedure. A method of correcting sample salinity effects was also proposed so that F⁻ concentrations were obtained using a calibration graph. The proposed method is simple: it requires no sample pretreatment aside from dilution. The following optimum conditions were established: background electrolyte (BGE), 5 mM 2,6-pyridinedicarboxylic acid (PDC) adjusted to pH 3.5 containing 0.03% m/v hydroxypropyl methylcellulose (HPMC); detection wavelength, 200 nm; vacuum (50 kPa) injection period of sample, 5 s (254 nL); and applied voltage, 23 kV with the sample inlet side as the cathode. The limit of detection (LOD, S/N = 3) and limit of quantification (LOQ, S/N = 10) for F⁻ reached 0.024 and 0.070 mg/L, respectively. The respective values of the relative standard deviation (RSD) of the peak area, peak height, and migration time for F⁻ were 2.5, 3.4, and 0.30%. The proposed method was applied for the determination of F⁻ in seawater samples collected from coastal waters of western Japan during August 26–28, 2014. Both results obtained using standard addition method and a calibration graph agreed with those obtained using a conventional spectrophotometric method.

中文翻译：

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瞬时等速电泳法在毛细管区电泳法测定海水中的氟化物

我们开发了毛细管区带电泳（CZE）与间接UV检测的氟化物的确定（F ^- ）在海水中使用的瞬时等速电泳（tITP），为上线浓缩程序。校正样品盐度作用的方法也被提出，以便那个F ^-使用校准图获得浓度。所提出的方法很简单：除了稀释外，不需要任何样品预处理。确定了以下最佳条件：背景电解质（BGE），5 mM 2,6-吡啶二甲酸（PDC）调节至pH 3.5，含有0.03％m / v羟丙基甲基纤维素（HPMC）；检测波长200 nm；样品的真空（50 kPa）注入时间，5 s（254 nL）; 然后以样品入口侧为阴极施加23 kV的电压。检测限（LOD，小号/ Ñ 定量= 3）和极限（LOQ，小号/ Ñ  = 10）与F ^-分别达到0.024和0.070 mg / L。相对标准偏差的峰面积，峰高，并与F迁移时间的（RSD）的相应值^-分别为2.5，3.4和0.30％。所提出的方法涂敷了用于F的判定^- 8月26日至28日在从西日本的沿海水域收集海水样品中，2014年使用标准添加法，并用所获得的那些使用常规的分光光度法商定的校准曲线得到的两个结果。