In this work, a molecularly imprinted monolithic column was synthesized by a facile procedure and was applied for specific recognition of domoic acid, an amnesic shellfish poison. The poly(4-vinylpyridine-co-ethylene glycol dimethacrylate) molecularly imprinted monolith was synthesized in a stainless steel column by in situ polymerization. Pentane-1,3,5-tricarboxylic acid was used as a dummy imprinting template instead of the highly toxic and expensive target molecule. It is the first time that a molecularly imprinted monolith is introduced for separation and detection of domoic acid. After optimizing the preparation conditions, the prepared imprinted monolith was systematically characterized and exhibited excellent stability and permeability as a HPLC stationary phase. The results of chromatographic analysis demonstrated that the molecularly imprinted monolith exhibited specific retention and selective recognition toward domoic acid, with an imprinted factor up to 3.77. Furthermore, the molecularly imprinted monolith was successfully applied for selective enrichment of domoic acid from biological samples.

中文翻译：

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分子印迹整料的制备和应用，用于对多摩酸的特异性识别

在这项工作中，通过简便的方法合成了分子印迹整体柱，并将其用于对遗忘性贝类毒物海藻酸的特异性识别。通过原位聚合在不锈钢柱中合成了分子印迹聚（4-乙烯基吡啶-共聚乙二醇二甲基丙烯酸乙二醇酯）的整料。戊烷1,3,5-三羧酸用作虚拟印迹模板，而不是剧毒且昂贵的靶分子。这是首次引入分子印迹整料用于分离和检测多摩酸。优化制备条件后，系统地表征了制备的印迹整体，并显示出出色的稳定性和渗透性，为HPLC固定相。色谱分析的结果表明，分子印迹的整体柱表现出对留存酸的特异性保留和选择性识别，印迹因子高达3.77。此外，分子印迹整料已成功应用于从生物样品中选择性富集海藻酸。