当前位置：
X-MOL 学术
›
J. Agric. Food Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Condensed Tannins from Longan Bark as Inhibitor of Tyrosinase: Structure, Activity, and Mechanism
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-22 00:00:00 , DOI: 10.1021/acs.jafc.7b05481 Wei-Ming Chai 1 , Qian Huang 1 , Mei-Zhen Lin 1 , Chong Ou-Yang 1 , Wen-Yang Huang 1 , Ying-Xia Wang 1 , Kai-Li Xu 1 , Hui-Ling Feng 2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-22 00:00:00 , DOI: 10.1021/acs.jafc.7b05481 Wei-Ming Chai 1 , Qian Huang 1 , Mei-Zhen Lin 1 , Chong Ou-Yang 1 , Wen-Yang Huang 1 , Ying-Xia Wang 1 , Kai-Li Xu 1 , Hui-Ling Feng 2
Affiliation
|
In this study, the content, structure, antityrosinase activity, and mechanism of longan bark condensed tannins were evaluated. The findings obtained from mass spectrometry demonstrated that longan bark condensed tannins were mixtures of procyanidins, propelargonidins, prodelphinidins, and their acyl derivatives (galloyl and p-hydroxybenzoate). The enzyme analysis indicated that these mixtures were efficient, reversible, and mixed (competitive is dominant) inhibitor of tyrosinase. What’s more, the mixtures showed good inhibitions on proliferation, intracellular enzyme activity and melanogenesis of mouse melanoma cells (B16). From molecular docking, the results showed the interactions between inhibitors and tyrosinase were driven by hydrogen bond, electrostatic, and hydrophobic interactions. In addition, high levels of total phenolic and extractable condensed tannins suggested that longan bark might be a good source of tyrosinase inhibitor. This study would offer theoretical basis for the development of longan bark condensed tannins as novel food preservatives and medicines of skin diseases.
中文翻译：
龙眼树皮中浓缩的单宁酸作为酪氨酸酶抑制剂：结构，活性和机理
本研究评估了龙眼树皮浓缩单宁的含量，结构，抗酪氨酸酶活性和机理。从质谱获得的结果表明，龙眼树皮缩合的单宁是原花青素，炔丙基炔醇，原炔菊醇及其酰基衍生物（没食子酰基和对羟基苯甲酸酯）的混合物。酶分析表明，这些混合物是有效，可逆的，混合的（竞争性为主）酪氨酸酶抑制剂。而且，混合物对小鼠黑色素瘤细胞的增殖，细胞内酶活性和黑色素生成均显示出良好的抑制作用（B 16）。从分子对接，结果表明抑制剂与酪氨酸酶之间的相互作用是由氢键，静电和疏水相互作用驱动的。此外，高含量的总酚和可提取的缩合单宁表明龙眼皮可能是酪氨酸酶抑制剂的良好来源。该研究为龙眼树皮浓缩单宁作为新型食品防腐剂和皮肤病药物的开发提供了理论依据。
更新日期：2018-01-22
中文翻译：
龙眼树皮中浓缩的单宁酸作为酪氨酸酶抑制剂：结构，活性和机理
本研究评估了龙眼树皮浓缩单宁的含量，结构，抗酪氨酸酶活性和机理。从质谱获得的结果表明，龙眼树皮缩合的单宁是原花青素，炔丙基炔醇，原炔菊醇及其酰基衍生物（没食子酰基和对羟基苯甲酸酯）的混合物。酶分析表明，这些混合物是有效，可逆的，混合的（竞争性为主）酪氨酸酶抑制剂。而且，混合物对小鼠黑色素瘤细胞的增殖，细胞内酶活性和黑色素生成均显示出良好的抑制作用（B 16）。从分子对接，结果表明抑制剂与酪氨酸酶之间的相互作用是由氢键，静电和疏水相互作用驱动的。此外，高含量的总酚和可提取的缩合单宁表明龙眼皮可能是酪氨酸酶抑制剂的良好来源。该研究为龙眼树皮浓缩单宁作为新型食品防腐剂和皮肤病药物的开发提供了理论依据。
京公网安备 11010802027423号