Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study. It was found that WBPPS mainly consisted of mannose, ribose, rhamnose, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.38:0.09:0.17:0.64:0.22:0.67:0.08:1.03, respectively. WBPPS was not affected by human saliva. The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded. Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.

中文翻译：

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人枸杞多糖提取物中人肠道菌群多糖体外模拟消化发酵

本文研究了从枸杞蜂花粉中提取的多糖的体外消化和发酵过程。发现WBPPS主要由甘露糖，核糖，鼠李糖，半乳糖醛酸，葡萄糖，半乳糖，木糖和阿拉伯糖组成，摩尔比分别为0.38：0.09：0.17：0.64：0.22：0.67：0.08：1.03。WBPPS不受人类唾液的影响。在模拟的胃液和小肠汁中，WBPPS的组分A（分子量1340 kDa）没有分解，而WBPPS的小组分B（分子量523 kDa）却被降解了。此外，发酵在体外显示，WBPPS可以显著提高了生产的短链脂肪酸和调制肠道菌群的组合物通过增加属的相对丰度普雷沃，Dialister，Megamonas，Faecalibacterium，和Alloprevotella和减小属数杆菌，梭状芽孢杆菌XlVa，Parabacteroides，大肠杆菌/志贺氏菌，Phascolarctobacterium，Parasutterella，梭菌属狭义，和梭杆菌。