Smartphone integrated optoelectrowetting (SiOEW) for on-chip sample processing and microscopic detection of water quality†,Lab on a Chip

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Smartphone integrated optoelectrowetting (SiOEW) for on-chip sample processing and microscopic detection of water quality†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-01-08 00:00:00 , DOI: 10.1039/c7lc01095h
Dongyue Jiang _{1,

2,

3,

4} , Seunguk Lee _{3,

4,

5} , Sung Woo Bae _{3,

4,

5} , Sung-Yong Park _{1,

2,

3,

4}

Affiliation

With the increasing capabilities and ubiquity of smartphones and their associated digital cameras, this study presents a smartphone integrated optoelectrowetting (SiOEW) device as a simple, portable tool capable of on-chip water sample preparation and microscopic detection of the target cells in water samples, which significantly reduce the detection time and the labor cost required for water quality monitoring. A commercially available smartphone is used as a low-intensity portable light source to perform optoelectrowetting (OEW)-based microfluidic operations such as droplet transportation, merging, mixing, and immobilization on a hydrophobic detection zone. Furthermore, a built-in smartphone camera allows on-chip microscopic detection of water quality with a 45× magnification. We have experimentally demonstrated that the SiOEW platform is able not only to automate the sample processing of marine water including the target algae cells (Amphiprora sp.) and staining reagents fluorescein diacetate (FDA) and 5-chloromethylfluorescein diacetate (CMFDA), but also detect the fluorescence signals emitted from the target cells in water samples and count their populations. Using the smartphone, the collected information (e.g. the location of the water sample collected and the time it was detected, the number of the target cells, etc.) can be rapidly and wirelessly shared with a central host such as an environmental regulation agency for real-time monitoring and further management of water quality.

中文翻译：

智能手机集成的光电湿法（SiOEW）用于片上样品处理和水质的微观检测†

随着智能手机及其相关数码相机功能的日益普及和普及，本研究提出了一种智能手机集成的光电湿润（SiOEW）设备，它是一种简单的便携式工具，能够进行芯片上的水样制备和对水样中的目标细胞进行微观检测，大大减少了检测时间和水质监测所需的人工成本。市售的智能手机用作低强度便携式光源，以执行基于光电湿法（OEW）的微流体操作，例如液滴运输，合并，混合和固定在疏水检测区域上。此外，内置的智能手机相机可在芯片上以45倍的放大率对水质进行微观检测。Amphiprora sp。）和染色试剂荧光素二乙酸酯（FDA）和5-氯甲基荧光素二乙酸酯（CMFDA），但也可以检测水样中靶细胞发出的荧光信号并计数其种群。使用智能手机，所收集的信息（例如，所收集的水样的位置以及检测到的时间，目标细胞的数量等）可以与中央主机（例如环境监管机构）快速无线共享实时监控和进一步管理水质。

更新日期：2018-01-08

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