Seeding Protein Crystallization with Cross-Linked Protein Crystals,Crystal Growth & Design

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Seeding Protein Crystallization with Cross-Linked Protein Crystals
Crystal Growth & Design ( IF 3.2 ) Pub Date : 2018-01-19 00:00:00 , DOI: 10.1021/acs.cgd.7b01536
Er-Kai Yan ₁ , Feng-Zhu Zhao ₁ , Chen-Yan Zhang ₁ , Xue-Zhou Yang ₁ , Miao Shi ₁ , Jin He ₁ , Ya-Li Liu ₁ , Yue Liu ₁ , Hai Hou _{1,

2} , Da-Chuan Yin _{1,

2}

Affiliation

Protein crystallization is of great importance because protein crystals have a number of different important applications, including large-scale purification of proteins, determination of protein structure, nanoparticle preparation, and theoretical studies of crystallization. An approach often used to efficiently crystallize proteins is the use of nucleants or seeds (small fragments of protein crystals) that can help increase the probability of protein crystallization. Due to the very positive effect that seeding has on protein crystallization, seeds are now widely accepted and utilized in practical protein crystallization. Here, we show that cross-linked protein crystals (CLPCs), which retain the crystal structure but are much more stable than non-cross-linked crystals, can also be used as a new type of seed for promoting protein crystallization. Seeding with CLPCs has effects on both the reproducibility and screening of protein crystals and could improve the optical perfection (well-defined facets) of protein crystals and the probability of obtaining protein crystals. In addition, the cross-linked protein crystals may reduce the concentration of protein molecules needed to obtain protein crystals. Furthermore, CLPCs are very stable in air, and no protective medium is necessary for the long-term storage of CLPCs. This feature makes the CLPC seeding method a potentially powerful technique in practical protein crystallization on either a laboratorial or an industrial scale.

中文翻译：

用交联的蛋白质晶体播种蛋白质结晶

蛋白质结晶非常重要，因为蛋白质晶体具有许多不同的重要应用，包括蛋白质的大规模纯化，蛋白质结构的确定，纳米颗粒的制备以及结晶的理论研究。通常用于有效结晶蛋白质的方法是使用成核剂或种子（蛋白质晶体的小片段），这可以帮助增加蛋白质结晶的可能性。由于播种对蛋白质结晶具有非常积极的作用，因此种子现已被广泛接受并用于实际的蛋白质结晶中。在这里，我们显示了保留了晶体结构但比未交联的晶体稳定得多的交联的蛋白质晶体（CLPC），也可用作促进蛋白质结晶的新型种子。CLPC的播种对蛋白质晶体的重现性和筛选都有影响，并且可以提高蛋白质晶体的光学完美性（明确定义的方面）以及获得蛋白质晶体的可能性。另外，交联的蛋白质晶体可降低获得蛋白质晶体所需的蛋白质分子的浓度。此外，CLPC在空气中非常稳定，长期保存CLPC不需要保护介质。此功能使CLPC播种方法成为实验室或工业规模的实用蛋白质结晶中潜在的强大技术。

更新日期：2018-01-19

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