In the current study, the feasibility of utilizing municipal secondary wastewater sludge for Polyhydroxyalkanoate (PHA) extraction was improved by optimization of various parameters (temperature, duration and concentration of sludge solids). Optimized process parameters resulted in PHA recovery of 0.605 g, significantly higher than un-optimized conditions. The characterization of PHA was carried out by GC–MS, FT-IR and NMR (¹H and ¹³C) spectroscopy. The PHA profile was found to be dominated by mcl PHA (58%) along with other diverse PHA. The results of the present study show rich diversity of PHA extracted from a raw material which is readily available at minimal cost. In conclusion, exploring the potential of wastes for production of bioplastics not only reduces the cost of bioplastic production, but also provides a sustainable means for waste management.

在当前研究中，通过优化各种参数（温度，持续时间和污泥固体浓度），提高了利用市政二次废水污泥提取聚羟基链烷酸酯（PHA）的可行性。优化的工艺参数可回收0.605 g PHA，明显高于未优化的条件。PHA的表征通过GC-MS，FT-IR和NMR（¹ H和¹³C）光谱学。发现PHA谱主要由mcl PHA（58％）以及其他各种PHA占据。本研究的结果表明，从原料中提取的PHA具有丰富的多样性，并且可以以最低的成本获得。总之，探索废物在生物塑料生产中的潜力，不仅可以降低生物塑料生产的成本，而且可以为废物管理提供一种可持续的手段。