Conductive poly(3,4-ethylenedioxythiophene) (PEDOT) composites have emerged as potential candidates for electrochemical biosensors, however, the sensing performance was mainly relied on their structure and composition. In this work, a novel three-dimensional (3D) structural nano-PEDOT-graphene aerogel (nano-PEDOT-GA) composite was designed via the in-situ polymerization of nano-PEDOT on porous 3D GA. SEM revealed that the introduction of GA could induce the growth of worm-like PEDOT. Subsequently, Au nanoparticles (AuNPs) were directed to nano-PEDOT-GA surface by electrodeposition method. Featuring the excellent biocompatibility, high specific surface area and good conductivity of AuNPs/nano-PEDOT-GA, a highly sensitive label-free immunosensor was developed using AuNPs/nano-PEDOT-GA as signal amplification platform for detecting prostate specific antigen (PSA). Under the optimal conditions, the fabricated immunosensor presented a good linear in the analytical range of 0.0001–50 ng mL⁻¹ with a low detection limit of 0.03 pg mL⁻¹ (S/N = 3), high selectivity against other serum interfering substance and good stability and reproducibility, which provides a promising platform for the clinical immunoassay of PSA.

导电聚（3,4-乙撑二氧噻吩）（PEDOT）复合材料已成为电化学生物传感器的潜在候选材料，但是其传感性能主要取决于其结构和组成。在这项工作中，通过在多孔3D GA上原位聚合纳米PEDOT，设计了一种新颖的三维（3D）结构纳米PEDOT-石墨烯气凝胶（nano-PEDOT-GA）复合材料。SEM表明，GA的引入可以诱导蠕虫状PEDOT的生长。随后，通过电沉积方法将金纳米颗粒（AuNPs）引导至纳米PEDOT-GA表面。AuNPs / nano-PEDOT-GA具有出色的生物相容性，高比表面积和良好的导电性，使用AuNPs / nano-PEDOT-GA作为检测前列腺特异性抗原（PSA）的信号放大平台，开发了一种高度灵敏的无标记免疫传感器。在最佳条件下，装配好的免疫传感器在0.0001–50 ng mL的分析范围内表现出良好的线性^-1具有0.03 pg mL ^-1的低检测限（S / N = 3），对其他血清干扰物质的高选择性以及良好的稳定性和可重复性，这为PSA的临床免疫分析提供了有希望的平台。