AbstractAn electrochemiluminescent (ECL) aptamer based assay is described for thrombin. It is based on the use of carbon dots (C-dots) placed on polydopamine nanospheres loaded with silver nanoparticles (PDANS@Ag) and with probe DNA (pDNA). The PDANS possess high specific surface and can load a large number of C-dots. The AgNPs, in turn, enhance the ECL emission of the C-dots. Platinum functionalized graphene (Gr-Pt) can connect capture DNA (cDNA). The ECL nanoprobe consisting of PDANS@Ag/C-dots was placed on a glassy carbon electrode modified with Gr-Pt/cDNA/BSA via hybridization between cDNA and pDNA. On applying voltages from −1.8 V to 0 V, a strong ECL signal is generated. If thrombin is added, it will bind to cDNA. This leads to the release of pDNA from the electrode surface and a decrease in ECL intensity. Response to thrombin is linear in the 1.0 fmol·L−1 to 5.0 nmol·L−1 concentration range, with a 0.35 fmol·L−1 detection limit. The assay is stable, repeatable and selective, which demonstrates its clinical applicability. Graphical abstractCarbon dots (C-dots) placed on polydopamine nanospheres loaded with silver nanoparticles (PDANS@Ag) for electrochemiluminescent (ECL) detection of thrombin.

中文翻译：

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使用固定在银装饰的聚多巴胺纳米球上的碳点进行基于适配体的电化学发光凝血酶测定

摘要描述了凝血酶的基于电化学发光 (ECL) 适配体的测定。它基于使用放置在负载有银纳米粒子 (PDANS@Ag) 和探针 DNA (pDNA) 的聚多巴胺纳米球上的碳点 (C-dots)。PDANS 具有高比表面积，可以加载大量 C 点。反过来，AgNPs 增强了 C 点的 ECL 发射。铂功能化石墨烯 (Gr-Pt) 可以连接捕获 DNA (cDNA)。由 PDANS@Ag/C 点组成的 ECL 纳米探针通过 cDNA 和 pDNA 之间的杂交放置在用 Gr-Pt/cDNA/BSA 修饰的玻璃碳电极上。在施加从 -1.8 V 到 0 V 的电压时，会生成强 ECL 信号。如果添加凝血酶，它将与 cDNA 结合。这导致 pDNA 从电极表面释放并降低 ECL 强度。对凝血酶的反应在 1.0 fmol·L-1 至 5.0 nmol·L-1 浓度范围内呈线性，检测限为 0.35 fmol·L-1。该测定是稳定的、可重复的和选择性的，这证明了其临床适用性。图形摘要碳点 (C-dots) 放置在负载有银纳米粒子 (PDANS@Ag) 的聚多巴胺纳米球上，用于凝血酶的电化学发光 (ECL) 检测。