The hydrophilic interaction liquid chromatography (HILIC) coupled to mass spectrometry was employed to study retention behavior of selected selenium compounds using two different HILIC stationary phases: silica and zwitterionic. Two organic solvents – acetonitrile and methanol – were compared as a component of mobile phase. Separation parameters such as a content of organic modifier, the eluent pH and inorganic buffer concentration were investigated. Based on all observations, methanol seems to be beneficial for the separation of studied compounds. The optimal HILIC separation method involved silica column and eluent composed of 85% MeOH and CH₃COONH₄ (8 mM, pH 7) was compared to RP method in terms of time of the single run, the separation efficiency and limit of detection.

使用质谱联用的亲水相互作用液相色谱法（HILIC），使用两种不同的HILIC固定相：硅胶和两性离子，研究所选硒化合物的保留行为。比较了两种有机溶剂（乙腈和甲醇）作为流动相的组成部分。研究了分离参数，如有机改性剂的含量，洗脱液的pH值和无机缓冲液的浓度。基于所有观察，甲醇似乎对分离所研究的化合物是有益的。最佳的HILIC分离方法包括硅胶柱和由85％MeOH和CH ₃ COONH ₄（8 mM，pH 7）组成的洗脱液，与RP方法的单次运行时间，分离效率和检测限相比。