Waste stabilization ponds (WSP) are one of the most common forms of wastewater treatment for smaller communities globally, but have poor phosphorus removal. It is known that WSP algae can accumulate polyphosphate within their cells in excess of that needed for cell function. If polyphosphate accumulation could be triggered at the higher range of WSP cell concentrations, phosphorus removal from domestic wastewater could be significantly improved. However, this phenomenon is sporadic and still not fully understood. With a view of building a fundamental understanding to underpin the engineering of a new phosphorus removal process, this paper examines eight previously untested variables that may influence the cellular phosphorus content of WSP biomass. Although calcium, magnesium, and potassium are key constituents of polyphosphate granules, the concentrations tested were not limiting to polyphosphate accumulation. While literature also pointed to inoculum characteristics as potentially having an impact, no significance was found in this research. Conversely, three important new triggers where identified that significantly (90% confidence) affected the cellular phosphorus content of WSP biomass. An increase in cellular phosphorus content was triggered by decreasing the organic load, or allowing the pH to increase as compared to pH control. By contrast, the presence of mixing decreased the phosphorus content of the WSP biomass.

废物稳定池（WSP）是全球较小社区的最常见废水处理形式之一，但除磷效果差。众所周知，WSP藻类在细胞内积累的多磷酸盐超过了细胞功能所需的多磷酸盐。如果可以在更高的WSP细胞浓度范围内触发多磷酸盐的积累，那么生活污水中的磷去除率将得到显着提高。但是，这种现象是零星的，仍然没有被完全理解。为了建立基础知识以支持新的除磷工艺的工程设计，本文研究了八个以前未经测试的变量，这些变量可能会影响WSP生物质的细胞磷含量。尽管钙，镁和钾是多磷酸盐颗粒的关键成分，测试的浓度不限于多磷酸盐的积累。尽管文献还指出接种物特性可能会产生影响，但在这项研究中未发现任何意义。相反，确定了三个重要的新触发因素，这些触发因素显着（90％置信度）影响了WSP生物质的细胞磷含量。与降低pH值相比，降低有机负荷或增加pH值可以触发细胞磷含量的增加。相反，混合的存在降低了WSP生物质的磷含量。确定了三个重要的新触发因素（90％置信度）显着影响了WSP生物质的细胞磷含量。与降低pH值相比，降低有机负荷或增加pH值可以触发细胞磷含量的增加。相反，混合的存在降低了WSP生物质的磷含量。确定了三个重要的新触发因素（90％置信度）显着影响了WSP生物质的细胞磷含量。与降低pH值相比，降低有机负荷或增加pH值可以触发细胞磷含量的增加。相反，混合的存在降低了WSP生物质的磷含量。