Fundamentals of rapid injection molding for microfluidic cell-based assays†,Lab on a Chip

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Fundamentals of rapid injection molding for microfluidic cell-based assays†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-01-08 00:00:00 , DOI: 10.1039/c7lc01052d
Ulri N. Lee _{1,

2,

3,

4} , Xiaojing Su _{1,

2,

3,

4} , David J. Guckenberger _{4,

5,

6,

7} , Ashley M. Dostie _{1,

2,

3,

4} , Tianzi Zhang _{1,

2,

3,

4} , Erwin Berthier _{1,

2,

3,

4} , Ashleigh B. Theberge _{1,

2,

3,

4,

8}

Affiliation

Microscale cell-based assays have demonstrated unique capabilities in reproducing important cellular behaviors for diagnostics and basic biological research. As these assays move beyond the prototyping stage and into biological and clinical research environments, there is a need to produce microscale culture platforms more rapidly, cost-effectively, and reproducibly. ‘Rapid’ injection molding is poised to meet this need as it enables some of the benefits of traditional high volume injection molding at a fraction of the cost. However, rapid injection molding has limitations due to the material and methods used for mold fabrication. Here, we characterize advantages and limitations of rapid injection molding for microfluidic device fabrication through measurement of key features for cell culture applications including channel geometry, feature consistency, floor thickness, and surface polishing. We demonstrate phase contrast and fluorescence imaging of cells grown in rapid injection molded devices and provide design recommendations to successfully utilize rapid injection molding methods for microscale cell-based assay development in academic laboratory settings.

中文翻译：

基于微流细胞的快速注射成型基础知识†

基于微型细胞的测定法已展示出独特的能力，可再现用于诊断和基础生物学研究的重要细胞行为。随着这些测定法超越原型阶段，进入生物学和临床研究环境，需要更快，更经济和可重复地生产微型培养平台。“快速”注塑成型有望满足这一需求，因为它以成本的一小部分实现了传统的大批量注塑成型的一些优势。然而，由于用于模具制造的材料和方法，快速注射成型具有局限性。在这里，我们通过测量细胞培养应用的关键特征（包括通道几何形状，具有一致性，地板厚度和表面抛光的特点。我们演示了在快速注射成型设备中生长的细胞的相衬和荧光成像，并提供了设计建议，以成功地在学术实验室环境中成功地将快速注射成型方法用于基于微小细胞的分析开发。

更新日期：2018-01-08

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