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SpyCatcher–SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells
Chemical Communications ( IF 4.9 ) Pub Date : 2018-01-08 00:00:00 , DOI: 10.1039/c7cc08880a
Xianliang Ke 1, 2, 3 , Yuan Zhang 3, 4, 5 , Fangliang Zheng 3, 6, 7 , Yan Liu 3, 4, 5 , Zhenhua Zheng 3, 4, 5 , Yi Xu 1, 2, 3 , Hanzhong Wang 3, 4, 5
Affiliation  

A non-invasive labelling strategy is proposed to label baculovirus via genetic insertion of a SpyTag into the viral glycoprotein, followed by specific conjugation with the SpyCatcher protein on modified quantum dots (QDs) through an isopeptide bond. The labelling method is convenient and efficient and shows little attenuation of viral infectivity. Therefore, it is a biologically compatible technique for tracking viral infection.

中文翻译:

SpyCatcher–SpyTag介导带有量子点的子代杆状病毒原位标记,用于追踪活细胞中的病毒感染

一种非侵入性的标记策略,提出了标签杆状病毒经由一个SpyTag的遗传插入病毒糖蛋白,通过异肽键,接着用改性量子点(QD)的抓间谍者蛋白特异性结合。标记方法方便有效,显示出很少的病毒感染力衰减。因此,它是用于追踪病毒感染的生物学兼容技术。
更新日期:2018-01-31
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