A luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the Golgi apparatus to lysosomes in living cells†,Chemical Science

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A luminescent aluminium salen complex allows for monitoring dynamic vesicle trafficking from the Golgi apparatus to lysosomes in living cells†
Chemical Science ( IF 7.6 ) Pub Date : 2018-01-08 00:00:00 , DOI: 10.1039/c7sc04498d
Juan Tang _{1,

2,

3,

4,

5} , Hao-Yan Yin _{1,

2,

3,

4,

5} , Jun-Long Zhang _{1,

2,

3,

4,

5}

Affiliation

The Golgi apparatus is well-known as the center of vesicle trafficking whose malfunction might cause the breakdown of overall cellular architecture and ultimately cell death. The development of fluorescent probes to not only precisely stain the Golgi apparatus but also monitor dynamic vesicle trafficking is of great significance. While fluorescent proteins and fluorescent lipid analogs have been reported, they are sometime limited by either overexpression and toxicity or lack of high selectivity, respectively. We herein report a novel approach based on metal-induced coordination between the phosphate anions of phospholipids and the metal center of a luminescent Alsalen complex AlL, which can in situ track membrane vesicle trafficking from the Golgi apparatus to the lysosomes in living cells. This work opens a new avenue for designing luminescent metal probes based on the Lewis acidity of metal ions and allows the use of metal ions with different charge states, polarities, and reactivities within a similar structural scaffold to expand coordination chemistry for biological studies.

中文翻译：

发光的铝salen复合物可以监测从高尔基体到活细胞中溶酶体的动态囊泡运输†

高尔基体众所周知是囊泡运输的中心，其故障可能导致整体细胞结构崩溃，最终导致细胞死亡。开发荧光探针不仅可以精确地对高尔基体染色，而且可以监测动态囊泡运输，这具有重要意义。尽管已经报道了荧光蛋白和荧光脂质类似物，但它们有时有时分别受到过表达和毒性或缺乏高选择性的限制。本文我们报告了一种新方法基于磷脂的磷酸根阴离子和发光Alsalen络合物的金属中心之间的金属诱导的协调所有，其可以原位跟踪从高尔基体到活细胞中的溶酶体的膜囊泡运输。这项工作为基于金属离子的路易斯酸度设计发光金属探针开辟了一条新途径，并允许在相似的结构支架中使用具有不同电荷态，极性和反应性的金属离子，从而扩展了用于生物学研究的配位化学。

更新日期：2018-01-08

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